Abstract
The primed in situ labelling (PRINS) technique is an alternative to in situ hybridization for chromosomal screening. We have developed a semi-automatic PRINS protocol, using a programmable thermocycler. The method has been successfully tested with specific primers for chromosomes, 13, 16, 18, 21, X and Y. Specific chromosome detection has been obtained on both metaphases and interphase nuclei. This suggests that PRINS may be a reliable technique for detecting aneuploidies and some chromosomal aberrations.
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References
Choo KH, Vissel B, Nagy A, Earle E, Kalitsis P (1991) A survey of the genomic distribution of alpha satellite DNA on all the human chromosomes, and derivation of a new consensus sequence. Nucleic Acids Res 19:1179–1182
Cremer T, Lichter P, Borden J, Ward DC, Manuelidis J (1988) Detection of chromosome aberrations in metaphase and interphase tumor cells by in situ hybridization using chromosome specific library probes. Hum Genet 80:235–246
Fuscoe JC, Collins CC, Pinkel D, Gray JW (1989) An efficient method for selecting unique-sequence clones from DNA libraries and its application to fluorescent staining of human chromosome 21 using in situ hybridization. Genomics 5:100–109
Gosden J, Hanratty D (1991) Comparison of sensitivity of three haptens in the PRINS (oligonucleotide primed in situ DNA synthesis) reaction. Technique 3:159–165
Gosden J, Hanratty D, Starling J, Mitchell A, Porteous D (1991) Oligonucleotide-primed in situ DNA synthesis (PRINS): a method for chromosome mapping, banding and investigation of sequence organization. Cytogenet Cell Genet 57:100–104
Hook EB (1985) The impact of aneuploidy upon public health: mortality and morbidity associated with human chromosome abnormalities. In: Dellarco VL, Voytek PE, Hollaender A (eds) Aneuploidy etiology and mechanisms. Plenum Press, New York, pp 7–33
Kallioniemi A, Kallioniemi OP, Sudar D, Rutovitz D, Gray JW, Waldman F, Pinkel D (1992) Comparative genomic hybridization for molecular cytogenetic analysis of solid tumors. Science 258:818–821
Klinger K, Landes G, Shook D, Harvey R, Lopez L, Locke P, Lerner T, Osathanondh R, Leverone B, Houseal T, Pavelka K, Dackowski W (1992) Rapid detection of chromosome aneuploidies in uncultured amniocytes by using fluorescence in situ hybridization (FISH). Am J Hum Genet 51:55–65
Koch JE, Hindkjaer J, Mogensen J, Kolvraa S, Bolund L (1991) An improved method for chromosome-specific labeling of satellite DNA in situ by using denatured double-standed DNA probes as primers in a primed in situ labelling (PRINS) procedure. Genet Anal Tech Appl 8:171–178
Lauritsen JG (1982) The cytogenetics of spontaneous abortion. Res Reprod 14:3–4
Lebo RV, Flandermeyer RR, Diukman R, Lynch ED, Lepercq JA, Golbus MS (1992) Prenatal diagnosis with repetitive in situ hybridization probes. Am J Med Genet 43:848–854
Nagesh Rao P, Hayworth R, Cox K, Grass F, Pettenati MJ (1993) Rapid detection of aneuploidy in uncultured chorionic villus cells using fluorescence in situ hybridization. Prenat Diagn 13:233–238
Pinkel D, Landegent J, Collins C, Fucsoe J, Segraves R, Lucas J, Gray J (1988) Fluorescence in situ hybridization with human chromosome-specific libraries: detection of trisomy 21 and translocations of chromosome 4. Proc Natl Acad Sci USA 85:9138–9142
Ried T, Landes G, Dackowski W, Klinger K, Ward DC (1992) Multicolor fluorescence in situ hybridization for the simultaneous detection of probe sets for chromosomes 13, 18, 21, X and Y in uncultured amniotic fluid cells. Hum Mol Genet 1:07–313
Trask B (1991) Gene mapping by fluorescence in situ hybridization. Curr Opinion Genet Dev 1:82–87
Zahed L, Murer-Orlando M, Vekemans M (1992) In situ hybridization studies for the detection of common aneuploidies in CVS. Prenat Diagn 12:483–493
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Pellestor, F., Girardet, A., Lefort, G. et al. Use of the primed in situ labelling (PRINS) technique for a rapid detection of chromosomes 13, 16, 18, 21, X and Y. Hum Genet 95, 12–17 (1995). https://doi.org/10.1007/BF00225066
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DOI: https://doi.org/10.1007/BF00225066