Abstract
We report the use of the PRimedIN Situ (PRINS) labelling technique for the direct estimation of disomy rates for various chromosomes in human sperm. The PRINS technique provides a rapid and reliable method for chromosome screening since specific labelling may be obtained in less than 2 h. In the present study, the disomy rates of chromosomes 2, 5, 9, 12 and 18 were investigated. The incidences of disomy are similar for all these chromosomes, ranging from 0.27% to 0.33%. These findings suggest an equal distribution of aneuploidies among autosomes in human sperm.
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Pellestor, F., Quennesson, I., Coignet, L. et al. Direct detection of disomy in human sperm by the PRINS technique. Hum Genet 97, 21–25 (1996). https://doi.org/10.1007/BF00218827
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DOI: https://doi.org/10.1007/BF00218827