Summary
The papillary muscle of the cat heart's right ventricle has not been studied previously with quantitative ultrastructural techniques despite its wide use for functional studies. This tissue was perfusion-fixed, processed for electron microscopy, and morphometric techniques were used to assess the ultrastructural characteristics of the papillary muscle as well as the working myocardial cells. The results of this study were that 73.5% of the papillary muscle was composed of muscle cells, 9.7% of blood vessels, and the remainder of interstitial connective tissue. In the muscle cell the volume fraction of mitochondria was 17.3%, that of myofibrils was 49.8%, and that of the nucleus was 2.0%. The mitochondria to myofibrils ratio was 0.36 and the surface to volume ratio was 0.309. In a quantitative ultrastructural comparison of perfusion and immersion fixed tissue it was found that significant differences in the volume density of the blood vessel lumen existed between the two groups. In addition, there were significant differences in the volume fraction of mitochondria and nucleus between perfusion-fixed and immersion-fixed muscle cells. A concurrent significant decrease between the two groups was also found for the ratio of mitochondria to myofibrils. The perfusion-fixed tissue can be considered to provide only normal baseline data for the papillary muscle of the right ventricle. These data are important as they can be used in future structure-function studies on normal and pathological heart tissue.
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References
Adomian GE, Laks MM, Billingham ME (1978) The incidence and significance of contraction bands in endomyocardial biopsies from normal human hearts. Am Heart J 95:348–351
Alpert NR, Hamrell BB, Halpern W (1974) Mechanical and biochemical correlates of cardiac hypertrophy. Circ Res (suppl II) 34–35:71–81
Anversa P, Olivetti G, Melissari M, Loud AV (1980) Stereological measurement of cellular and subcellular hypertrophy and hyperplasia in the papillary muscle of adult rat. J Mol Cell Cardiol 12:781–795
Baandrup U, Olsen EGJ (1981) Critical analysis of endomyocardial biopsies from patients suspected of having cardiomyopathy. I. Morphological and morphometric aspects. Br Heart J 45:475–486
Baandrup U, Florio RA, Roters F, Olsen EGJ (1981a) Electron microscopic investigation of endomyocardial biopsy samples in hypertrophy and cardiomyopathy. A semiquantitative study in 48 patients. Circulation 63:1289–1298
Baandrup U, Florio RA, Rehahn M, Richardson PJ, Olsen EGJ (1981b) Critical analysis of endomyocardial biopsies from patients suspected of having cardiomyopathy. II. Comparison of histology and clinical/haemodynamic information. Br Heart J 45:487–493
Colgan JA, Lazarus ML, Sachs HG (1978) Postnatal development of the normal and cardiomyopathic Syrian hamster heart: A quantitative electron microscopic study. J Mol Cell Cardiol 10:43–54
Cooper G, Tomanek RJ, Ehrhardt JC, Marcus ML (1981) Chronic progressive pressure overload of the cat right ventricle. Circ Res 48:488–497
David H (1979) Some recent results of the quantitative characterization of heart muscle cells. Z mikrosk-anat Forsch. 93:113–137
Fawcett DW, McNutt MS (1969) The ultrastructure of the cat myocardium. I. Ventricular papillary muscle. J Cell Biol 42:1–45
Ferrans VJ, Roberts WC (1978) Myocardial biopsy: A useful diagnostic procedure or only a research tool? Am J Cardiol 41:965–967
Fleischer M, Wippo W, Themann H, Achatzy R-S (1980) Ultrastructural morphometric analysis of human myocardial left ventricles with mitral insufficiency. A comparison with normally loaded and hypertrophied human left ventricles. Virchows Arch Pathol Anat 389:205–210
Frank JS, Langer GA (1974) The myocardial interstitium: Its structure and its role in ionic exchange. J Cell Biol 60:586–601
Hamrell BB, Alpert NR (1977) The mechanical characteristics of hypertrophicd rabbit cardiac muscle in the absence of congestive heart failure. The contractile and series elastic elements. Circ Res 40:20–25
Houser SR, Freeman AR, Jaeger JM, Breisch EA, Coulson RL, Carey R, Spann JF (1981) Resting potential changes associated with Na-k pump in failing heart muscle. Am J Physiol 240:H168-H176
Legato MJ (1976) Ultrastructural changes during normal growth in the dog and rat ventricular myofiber. In: M Lieberman, T Sano (ed) Developmental and physiological correlates of cardiac muscle. Raven Press NY, pp 249–274
Legato MJ (1979a) Cellular mechanisms of normal growth in the mammalian heart. I. Qualitative and quantitative features of ventricular architecture in the dog from birth to five months of age. Circ Res 44(2):250–252
Legato MJ (1979b) Cellular mechanism of normal growth in the mammalian heart. II. A quantitative and qualitative comparison between the right and left ventricular myocytes in the dog from birth to five months of age. Circ Res 44:263–279
Mall G, Reinhard H, Stopp D, Rossner JA (1978) An effective morphometric method for electron microscopic studies on papillary muscles. Virchows Arch Pathol Anat 379:219–228
McCallister LP, Trapukdi S, Neely JR (1979) Morphometric observations on the effects of ischemia in the isolated perfused rat heart. J Mol Cell Cardiol 11:619–630
Natarajan G, Bove AA, Coulson RL, Carey RA, Spann JF (1979) Increased passive stiffness of short-term pressure-overload hypertrophied myocardium in cat. Am J Physiol 237:H676–680
Page E, McCallister LP, Power B (1971) Stereological measurements of cardiac ultrastructures implicated in excitation-contraction coupling. Proc Natl Acad Sci USA 68:1465–1466
Polimeni PI (1974) Extracellular space and ionic distribution in rat ventricle. Am J Physiol 227:676–683
Reynolds ES (1963) The use of lead citrate at high pH as an electron opaque stain in electron microscopy. J Cell Biol 17:208–212
Schwarz F, Shaper J, Kittstein D, Flameng W, Walter P, Schaper W (1981) Reduced volume fraction of myofibrils in myocardium of patients with decompensated pressure overload. Circulation 63:1299–1304
Sekiguchi M, Haze K, Hiroe M, Konno S, Hirosawa K (1978) Interrelation of left ventricular function and myocardial ultrastructure as assessed by endomyocardial biopsy: Comparative study of hypertrophic and congestive cardiomyopathies. Recent Adv Stud Cardiac Struct Funct 12:327–334
Sheridan DJ, Cullen MJ, Tynan MJ (1977) Postnatal ultrastructural changes in the cat myocardium: A morphometric study. Cardiovasc Res 11:536–540
Singh S, White FC, Bloor CM (1981) Myocardial morphometric characteristics in swine. Circ Res 49:434–441
Spurr AR (1969) A low-viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruct Res 26:31–43
Thomsen HK, Torp A (1979) Value and limitations of human myocardial biopsy. J Mol Cell Cardiol 11:467–475
Tomanek RJ (1979) The role of prevention or relief of pressure overload on the myocardial cell of the spontaneously hypertensive rat. A morphometric and stereologic study. Lab Invest 40:83–91
Tomanek RJ, Karlsson WL (1973) Myocardial ultrastructure of young and senescent rats. J Ultrastruct Res 42:201–220
Tomanek RJ, Davis JW, Anderson SC (1979) The effects of alphamethyldopa on cardiac hypertrophy in spontaneously hypertensive rats: ultrastructural, stereological and morphometric analysis. Cardiovasc Res 13:173–182
Watson ML (1958) Staining of tissue sections for electron microscopy with heavy metals. J Biophys Biochem Cytol 4:475–478
Weibel ER (1969) Stereological principles for morphometry in electron microscopic cytology. Int Rev Cytol 26:235–303
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Marino, T.A., Houser, S.R., Martin, F.G. et al. An ultrastructural morphometric study of the papillary muscle of the right ventricle of the cat. Cell Tissue Res. 230, 543–552 (1983). https://doi.org/10.1007/BF00216200
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DOI: https://doi.org/10.1007/BF00216200