Abstract
Human chromosome 6 has been subdivided by chromosome microdissection into 14 unique regions. Following microdissection, polymerase chain reaction (PCR) amplification of dissected DNA was performed using a universal primer to generate subregion-specific probes that provided complete coverage of chromosome 6. All 16 microdissections have been regionally assigned along chromosome 6 by fluorescence in situ hybridization (FISH) using biotin-labeled dissected DNA hybridized to G-banded normal metaphase chromosomes. These probes can be used as region-specific paints to generate unique “bar codes” and for analysis of chromosome alterations involving chromosome 6 that are unidentifiable by conventional banding analysis.
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Guan, XY., Meltzer, P.S., Burgess, A.C. et al. Coverage of chromosome 6 by chromosome microdissection: generation of 14 subregion-specific probes. Hum Genet 95, 637–640 (1995). https://doi.org/10.1007/BF00209479
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DOI: https://doi.org/10.1007/BF00209479