Abstract
We have produced physical maps of the proximal part of Xq22, containing the Bruton's tyrosine kinase (BTK) and α-galactosidase A (GLA) gene loci, using long range physical mapping techniques and yeast artificial chromosomes (YACs). These maps reveal five previously unidentified CpG islands which could indicate the presence of other genes in this region.
Similar content being viewed by others
References
Allen RC, Belmont JB (1992) Dinucleotide repeat polymorphism at the DXS178 locus. Hum Mol Genet 1: 216
Anand R, Villasante A, Tyler-Smith C (1989) Construction of yeast artificial chromosome libraries with large inserts using fractionation by pulsed field gel electrophoresis. Nucleic Acids Res 17: 3425–3433
Barker DF, Hostikka SL, Zhou J, Chow LT, Oliphant AR, Gerken SC, Gregory MC, Skolnick MH, Atkin CL, Tryggvason K (1990) Identification of the mutation in the COLAS collagen gene in Alport syndrome. Science 248: 1224–1227
Barker DF, Fain PR, Goldgar DE, Dietz-Band JN, Turco AE, Kashtan CE, Gregory MC, Tryggvason K, Skolnick MH, Atkin CL (1991) High density genetic and physical mapping of DNA markers near the X-linked Alport Syndrome locus: definition and use of flanking polymorphic markers. Hum Genet 88: 189–194
Bernstein HS, Bishop DF, Astrin KH, Kornreich R, Eng CM, Sakuraba H, Desnick RJ (1989) Fabry disease: Six gene rearrangements and an exonic point mutation in the alpha galactosidase A gene. J Clin Invest 83: 1390–1399
Bird A (1986) CpG rich islands and the function of DNA methylation. Nature 321: 209–213
Collins FS (1992) Positional cloning: Let's not call it reverse anymore. Nature Genet 1: 3–6
Davies KE, Mandel J-L, Monaco AP, Nussbaum RL, Willard HF (1991) Report of the committee on the genetic constitution of the X chromosome. Cytogenet Cell Genet 58: 853–966
Hudson LD, Puckett C, Berndt J, Chan J, Gencic S (1989) Mutation of the proteolipid protein gene PLP in a human X-chromosome linked myelin disorder. Proc Natl Acad Sci USA 86: 8128–8131
Larin Z, Monaco A, Lehrach H (1991) Yeast artificial chromosome libraries containing large inserts from mouse and human DNA. Proc Natl Acad Sci USA 88: 4123–4127
Lovering R, Middleton-Price HR, O'Reilly M-AJ, Genet SA, Parkar M, Sweatman AK, Bradley LD, Alterman LA, Malcolm S, Morgan G, Levinsky RJ, Kinnon C (1993a) Genetic linkage analysis identifies new proximal and distal flanking markers for the X-linked agammaglobulinemia gene locus, refining its localization in Xq22. Hum Mol Genet 2: 139–141
Lovering R, Sweatman AK, O'Reilly M-AJ, Genet S, Middleton-Price H, Malcolm S, Levinsky RJ, Kinnon C (1993b) Physical mapping identifies DXS265 as a useful genetic marker for carrier detection and prenatal diagnosis of X-linked agammaglobulinemia. Hum Genet 91: 178–180
O'Reilly M-AJ, Alterman LA, Malcolm S, Levinsky RJ, Kinnon C (1993a) Identification of CpG islands around the DXS178 locus in Xq22, near to the X-linked agammaglobulinemia locus. Hum Genet 90: 275–278
O'Reilly M-AJ, Sweatman AK, Bradley LD, Alterman LA, Lovering R, Malcolm S, Levinsky RJ, Kinnon C (1993b) Isolation and mapping of discrete DXS101 loci in Xq22 near to the X linked agammaglobulinemia gene locus. Hum Genet 91: 605–608
Parolini O, Hejtmancik JF, Allen RC, Belmont JW, Lassiter GL, Henry MJ, Barker DF, Conley ME (1993) Linkage analysis and physical mapping near the gene for X-linked agammaglobulinemia at Xq22. Genomics 15: 342–349
Riley J, Butler R, Ogilvie D, Finniear R, Jenner D, Powell S, Anand R, Smith JC, Markham AF (1990) A novel, rapid method for the isolation of terminal sequences from yeast artificial chromosome (YAC) clones. Nucleic Acids Res 18: 2887–2890
Saugier-Vebere P, Munnich A, Bonneau D, Rozet J-M, Le Merrer M, Gil R, Boespflug-Tanguy O (1994) X-linked spastic paraplegia and Pelizaeus-Merzbacher disease are allelic disorders at the proteolipid protein locus. Nature Genet 6: 257–262
Tsukada S, Saffron DC, Rawlings DJ, Parolini O, Allen RC, Klisak I, Sparkes RS, Kubagawa H, Mohandas T, Quan S, Belmont JW, Cooper MD, Conley ME, Witte ON (1993) Deficient expression of a B cell cytoplasmic tyrosine kinase in human X-linked agammaglobulinemia. Cell 72: 279–290
Vetrie D, Bobrow M, Harris A (1993a) Construction of a 5.2-megabase physical map of the human X chromosome at Xq22 using pulsed field gel electrophoresis and yeast artificial chromosomes. Genomics 15: 631–642
Vetrie D, Vorechovsky I, Sideras P, Holland J, Davies A, Flinter F, Hammarstrom L, Kinnon C, Levinsky R, Bobrow M, Smith CIE, Bentley D (1993b) The gene involved in X-linked agamaglobulinemia (XLA) is a member of the src family of protein tyrosine kinases. Nature 361: 226–233
Vetrie D, Kendall E, Coffey A, Hassock S, Collins J, Todd C, Lehrach H, Bobrow M, Bentley DR, Harris A (1994) A 6.5 Mb yeast artificial chromosome contig incorporating 33 DNA markers on the human X chromosome at Xq22. Genomics 19: 42–47
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sweatman, A.K., Bradley, L.A.D., Lovering, R.C. et al. Physical mapping in the region of the Bruton's tyrosine kinase and α-galactosidase A gene loci in proximal Xq22. Hum Genet 94, 624–628 (1994). https://doi.org/10.1007/BF00206955
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00206955