Abstract
The X-linked agammaglobulinaemia (XLA) gene locus has previously been mapped to Xq22 in genetic linkage studies. The DXS101 locus has shown no recombinations with XLA in the ten informative meioses investigated so far. The DXS101 sequence, recognised by the cX52.5 plasmid, is moderately repeated in Xq22. We have isolated cosmids which contain this sequence; two copies of which have been found to lie near DXS178 and XLA, and a third copy which lies near the PLP gene, distal to these loci. We have used the cosmids to generate probes which should be of use for RFLP analysis, and thus in both prenatal diagnosis and carrier testing for XLA, and in constructing a genetic map of this region. These probes will also be used to complement the genetic map in the construction of a complete physical map of Xq22.
This is a preview of subscription content, log in via an institution to check access.
References
Barker DF, Fain PR, Goldgar DE, Dietz-Band JN, Turco AE, Kashtan CE, Gregory MC, Tryggvason K, Skolnick MH, Atkin CL (1991) High density genetic and physical mapping of DNA markers near the X-linked Alport syndrome locus: definition and use of flanking polymorphic markers. Hum Genet 88:189–194
Davies KE, Mandel J-L, Monaco AP, Nussbaum RL, Willard HF (1991) Report of the Committee on the Genetic Constitution of the X Chromosome. Cytogenet Cell Genet 58:853–966
Guioli S, Arveiler B, Bardoni B, Notarangelo LD, Panina P, Duse M, Ugazio A, Oberlé I, de Saint Basile G, Mandel JL, Camerino G (1989) Close linkage of probe p212 (DXS178) to X-linked agammaglobulinemia. Hum Genet 84:19–21
Hofker MH, Bergen AAB, Skraastad MI, Carpenter NJ, Veenema H, Conner JM, Bakker E, van Ommen GJB, Pearson PL (1987) Efficient isolation of X chromosome-specific single-copy probes from a cosmid library of a human X/hamster hybrid-cell line: mapping of new probes close to the locus for X-linked mental retardation. Am J Hum Genet 40:312–328
Kwan S-P, Terwilliger J, Parmley R, Raghu G, Sandukyl LA, Ott J, Ochs H, Wedgwood R, Rosen R (1990) Identification of a closely linked DNA marker, DXS178, to further refine the X-linked agammaglobulinemia locus. Genomics 6:238–242
Kwan S-P, Terwilliger J, Hagemann T, Ott J, Ochs H, Wedgwood R, Rosen F (1991) Linkage mapping of closely linked DNA markers for X-linked agammaglobulinemia. Cytogenet Cell Genet 58:A27489
Lovering R, Sweatman AK, O'Reilly M-AJ, Genet S, Middleton-Price H, Malcolm S, Levinsky RJ, Kinnon C (1993) Physical mapping identifies DXS265 as a useful genetic marker for carrier detection and prenatal diagnosis of X-linked agammaglobulinemia. Hum Genet 91:178–180
Malcolm S, de Saint Basile G, Arveiler B, Lau YL, Szabo P, Fischer A, Griscelli C, Debre M, Mandel JL, Callard RE, Robertson ME, Goodship JA, Pembrey ME, Levinsky RJ (1987) Close linkage of random DNA fragments from Xq21.3–22 to X-linked agammaglobulinemia (XLA). Hum Genet 77:172–174
Nizetic D, Zehetner G, Monaco AP, Gellen L, Young BD, Lehrach H (1991) Construction, arraying, and high-density screening of large insert libraries of human chromosome X and 21: their potential use as reference libraries. Proc Natl Acad Sci USA 88:3233–3237
O'Reilly M-AJ (1991) Physical and genetic mapping of the X-linked agammaglobulinemia locus. PhD Thesis, University of London, UK
O'Reilly M-AJ, Alterman LA, Malcolm S, Levinsky RJ and Kinnon C. (1992) Identification of CpG-islands around the DXS178 ocus in Xq22, near to the X-linked agammaglobulinemia locus. Hum Genet 90:275–278
O'Reilly M-AJ, Alterman LA, Zijlstra J, Malcolm S, Levinsky RJ, Kinnon C (1993) Pulsed field gel electrophoresis and radiation hybrid mapping analyses enable the ordering of eleven DNA loci in Xq22. Genomics 15:275–282
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
O'Reilly, MA.J., Sweatman, A.K., Bradley, L.D. et al. Isolation and mapping of discrete DXS101 loci in Xq22 near the X-linked agammaglobulinaemia gene locus. Hum Genet 91, 605–608 (1993). https://doi.org/10.1007/BF00205088
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00205088