Abstract
l-Lysine is known to stimulate production of β-lactam antibiotics byStreptomyces clavuligerus via provision of the lysine breakdown product,l-α-aminoadipic acid, which is a limiting precursor. Previous investigations utilized levels of 10–20 mMl-lysine as an addition to chemically-defined media resulting in 50–100% improvement in antibiotic production. We were surprised to note that as the concentration was further increased, the organism responded by producing even higher titers of antibiotics. The optimum concentration of 100 mMl-lysine yielded an approximate 500% increase in production with only minor effects on growth.dl- andd-lysine also exerted enhancements suggesting the presence of a lysine racemase or some other route fromd-lysine tol-α-aminoadipate in this organism;d-lysine was considerably less potent thandl- orl-lysine.
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Fang, A., Keables, P. & Demain, A.L. Unexpected enhancement of β-lactam antibiotic formation inStreptomyces clavuligerus by very high concentrations of exogenous lysine. Appl Microbiol Biotechnol 44, 705–709 (1996). https://doi.org/10.1007/BF00178606
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DOI: https://doi.org/10.1007/BF00178606