Abstract
Effects of histamine, histamine agonists and antagonists on steady state current in principal neurons and interneurons were investigated in thin slices from the olfactory bulb of newborn rabbits with the nystatin perforated patch-clamp technique and local pipette application. No change in steady state current was observed in mitral cells. In most of the periglomerular, juxtaglomerular and granular cells, however, H1-receptor activation caused an outward current; a similar effect, but mostly not on the same neurons was elicited by 8-bromo-cyclic-AMP. These currents were reversed at the potassium equilibrium potential and blocked by apamin and therefore probably represent calcium sensitive potassium currents. H2-receptor activation caused an inward current which also reversed at the potassium equilibrium potential, indicating block of a potassium current. Specific H3-receptor activation and cyclic GMP were ineffective. Histamine usually caused a combined effect beginning with an inward current. Histaminergic neurons fire with changes in behavioural state and can, by the described mechanisms, markedly influence signal processing in the olfactory bulb.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Arrang JM, Garbarg M, Schwartz JC (1987) Auto-inhibition of histamine synthesis mediated by presynaptic H3 receptors. Neuroscience 23:149–157
Bufler J, Zufall F, Franke C, Hatt H (1992a) Patch-clamp recordings of spiking and nonspiking interneurons from rabbit olfactory bulb slices: membrane properties and ionic currents. J Comp Physiol A 170:145–152
Bufler J, Zufall F, Franke C, Hatt H (1992b) Patch-clamp recordings of spiking and nonspiking interneurons from rabbit olfactory bulb slices: GABA and other transmitter receptors. J Comp Physiol A 170:153–159
Bufler J, Opitz T, Hatt H (1993) Electrophysiological and morphological properties of granule cells: patch-clamp recordings of newborn rabbit olfactory bulb slices. Neurosci Lett 161:129–132
Carbone E, Lux HD (1987) Kinetics and selectivity of a low-voltage-activated calcium current in chick and rat sensory neurons. J Physiol 386:547–570
Edwards FA, Konnerth A, Sakmann B, Takahashi T (1989) A thin slice preparation for patch clamp recordings from neurons of the mammalian central nervous system. Pflügers Arch 41:600–612
Ericson H, Watanabe T, Köhler CH (1987) Morphological analysis of the tuberomammillary nucleus in the rat brain: delinaeation of subgroups with antibody against L-histidine decarboxylase as a marker. J Comp Neurol 263:1–24
Greene RW, Haas HL (1990) Effects of histamine on dentate granule cells in vitro. Neuroscience 34:299–303
Haas HL (1984) Histamine potentiates neuronal excitation by blocking a calcium dependent potassium conductance. Agents and Actions 14:534–537
Haas HL (1992) Electrophysiology of histamine-receptors. In: Schwartz JC, Haas HL (eds) The histamine receptor. Wiley Liss Inc, New York, pp 161–177
Haas HL, Greene RW (1986) Effects of histamine on hippocampal pyramidal cells of the rat in vitro. Exp Brain Res 62:123–130
Haas HL, Konnerth A (1983) Histamine and noradrenaline decrease calcium-activated potassium conductance in hippocampal pyramidal cells. Nature 302:432–434
Haas HL, Jefferys JGR, Slater NT, Carpenter DO (1984) Modulation of low Ca induced field bursts in the hippocampus by monoamines and cholinomimetics. Pflügers Arch 400:28–33
Haas HL, Reiner PB, Greene RW (1991) Histaminergic and histaminoceptive neurons: electrophysiological studies in vertebrates. In: Watanabe T, Wada H (eds) Histaminergic neurons: morphology and function. CRC Press, Boston, pp 196–207
Hamill OP, Marty A, Neher E, Sakmann B, Sigworth FJ (1981) Improved patch clamp techniques for high-resolution current recordings from cells and cell free patches. Plügers Arch 391:85–100
Horn R, Marty A (1988) Muscarinic activation of ionic currents measured by a new whole-cell recording method. J Gen Physiol 92:145–159
Hughes M; Romey G, Duval D, Vincent, JP, Lazdunski M (1982) Apamin as a selective blocker of the calcium dependent potassium channel in neuroblastoma cells: voltage clamp and biochemical characterization of the toxin receptor. Proc Natl Acad Sci USA 79:1308–1312
Inagaki N, Yamatodani A, Ando-Yamamoto M, Tohyama M, Watanabe T, Wada H (1988) Organization of histaminergic fibers in the rat brain. J Comp Neurol 273:283–300
Lin JS, Sakai K, Jouvet M (1988) Evidence for histaminergic arousal mechanisms in the hypothalamus of cat. Neuropharmacology 27:111–122
McCormick DA (1992) Neurotransmitter actions in the thalamus and cerebral cortex and their role in neuromodulation of thalamocortical activity. Progr Neurobiol 39:337–388
Monnier M, Sauer R, Hatt AM (1970) The activating effect of histamine on the central nervous system. Int Rev Neurobiol 12:265–305
Mori K, Kishi K, Ojima H (1983) Distribution of dendrites of mitral, displaced mitral, tufted and granule cells in the rabbit olfactory bulb. J Comp Neurol 219:339–355
Orona E, Scott J, Rainer E (1983) Different granule cell populations innervate superficial and deep regions of the external plexiform layer in rat olfactory bulb. J Comp Neurol 217:227–237
Panula P, Pirvola U, Auvinen S, Airaksinen MN (1989) Histamine-immunoreactive nerve fibers in the brain. Neuroscience 28:585–610
Prell GD, Green JP (1986) Histamine as a neuroregulator. Ann Rev Neurosci 9:209–254
Rae JL, Fernandez J (1991) Perforated patch recordings in physiology. NIPS 6:273–277
Rae JL, Cooper K, Gates P, Watsky M (1991) Low access resistance perforated patch recordings using amphotericin B. J Neurosci Methods 37:15–26
Reiner PB, Kamondi A (1994) The mechanisms of antihistamine induced sedation in the human brain: H1 receptor activation reduces a background leakage potassium current. Neuroscience 59:579–588
Schwartz JC (1988) Histamine receptors in brain. ISI Atlas of Science, Pharmacology, pp 185–189.
Schwartz JC, Arrang JM, Garbarg M (1986) Three classes of histamine receptors in the brain. TIPS 7:24–28
Schwartz JC, Arrang JM, Garbarg M, Pollard H, Ruat M (1991) Histaminergic transmission in the mammalian brain. Physiol Rev 71:1–51
Scott JW, Harrison TA (1987) The olfactory bulb: Anatomy and Physiology. In: Finger T, Silver WL (eds) Neurobiology of taste and smell. John Wiley, New York, pp 151–178
Shepherd GM, Greer CA (1990) Olfactory bulb. In: Sheperd GM (ed) The Synaptic Organization of the Brain. Oxford University Press, New York, pp 132–169
Stuart GJ, Dodt HU, Sakmann B (1993) Patch-clamp recordings from the soma and dendrites of neurons in brain slices using infrared video microscopy. Pflügers Arch 423:511–518
Vorobjev S, Sharanova IN, Walsh IB, Haas HL (1993) Histamine potentiates N-methyl-D-aspartate responses in acutely isolated hippocampal neurons. Neuron 11:837–844
Wada H, Inagaki N, Yamatodani A, Watanabe T (1991) Is the histaminergic neuron system a regulatory center for whole-brain activity? TINS 14:415–418
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Jahn, K., Haas, H.L. & Hatt, H. Patch clamp study of histamine activated potassium currents on rabbit olfactory bulb neurons. Naunyn-Schmiedeberg's Arch Pharmacol 352, 386–393 (1995). https://doi.org/10.1007/BF00172775
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00172775