Summary
Lipases A and B from C.rugosa have been chemically modified with octanoyl chloride or polyethylene glycol (molecular weight: 750 or 5000). Although the hydrolytic activity was diminished, modification gave rise in some cases to preparations with increased synthetic activity in hexane. Furthermore, PEG5000-lipases A and B were significantly more stable (up to 20-fold) at alkaline pH values than the native enzymes.
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Bardsley, G.W (1993). SIMFIT: “A package for simulation, curve-fitting and statistical analysis in the life sciences”, Manchester University, U.K.
Basri, M., Salleh, A.B., Ampon, K., Yunus, W.M.Z. and Razak, C.N.A. (1991). Biocatalysis 4, 313–317.
Chulalaksananukul, W., Condoret, J.S., Delorme, P. and Willemot, R.M. (1990). FEBS Lett. 276, 181–184.
Habbeb, A.F.S.A (1966). Anal. Biochem. 14, 328–336.
Kawasaki, Y., Takahashi, Y., Nakatani, M., Murakami, M., Dosako, S. and Okai, H. (1994). Biosci. Biotech. Biochem. 58, 512–516.
Khmelnitsky, Y.L., Welch, S.H., Clark, D.S. and Dordick, J.S. (1994). J. Am. Chem. Soc. 116, 2647–2648.
Ljunger, G., Adlercreutz, P. and Mattiasson, B. (1992). Biocatalysis 7, 279–288.
Lotti, M., Tramontano, A., Longhi, S., Fusetti, F., Brocca, S., Pizzi, E. and Alberghina, L. (1994). Protein Eng. 7, 531–535.
Nishimura, H., Takahashi, K., Sakurai, K., Fujinuma, K., Inamura, Y., Ooba, M. and Inada, Y. (1983). Life Sci. 33, 1467–1473.
Otero, C., Pastor, E. and Ballesteros, A. (1990). Appl. Biochem. Biotechnol. 26, 35–44.
Pasta, P, Riva, S. and Carrea, G. (1988). FEBS Lett. 236, 329–332.
Peled, N. and Krenz, M.C. (1981). Anal. Biochem. 112, 219–222.
Plou, F.J. and Ballesteros, A. (1994). FEBS Lett. 339, 200–204.
Rúa, M.L., Díaz-Mauriño, T., Fernández, V.M., Otero, C. and Ballesteros, A. (1993). Biochim. Biophys. Acta 1156, 181–189.
Rúa, M.L. and Ballesteros, A. (1994). Biotechnol. Tech. 8, 21–26.
Schultz, A.M., Henderson, L.E. and Oroszlan, S. (1988). Annu. Rev. Cell Biol. 4, 611–647.
Shami, E.Y., Rothstein, A. and Ramjeesingh, M. (1989). Trends Biotechnol. 7, 186–190.
Takahashi, K., Saito, Y. and Inada, Y. (1988). J. Am. Oil Chem. Soc. 65, 911–916.
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Calvo, M.V., Plou, F.J., Pastor, E. et al. Effect of chemical modification of isoenzymes A and B from C. rugosa on their activity and stability. Biotechnol Lett 17, 171–176 (1995). https://doi.org/10.1007/BF00127983
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DOI: https://doi.org/10.1007/BF00127983