Abstract
A simple and rapid pea regeneration procedure was developed. An average of up to 20 shoots formed from hypocotyl explants of cvs. Sugar Ann and Patriot cultured on Murashige and Skoog basal medium supplemented with 0.5 or 1.0 μM thidiazuron (N-phenyl-N′-1,2,3-thiadiazol-5′-ylurea). Hypocotyls of Puget and Sugar Daddy did not respond. Regenerated shoots rooted rapidly when cultured on Murashige and Skoog basal medium containing either 2.0 μM α-naphthaleneacetic acid or 1.0–2.0 μM indole-3-butyric acid. Seeds were harvested from regenerated plants after only 9–11 weeks.
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Abbreviations
- BAP:
-
6-benzyladenine
- 2,4-d :
-
2,4-dichlorophenoxy acetic acid
- GA3 :
-
gibberellic acid
- IBA:
-
indole-3-butyric acid
- MS:
-
Murashige and Skoog (1962) medium
- NAA:
-
α-naphthaleneacetic acid
- TDZ:
-
thidiazuron (N-phenyl-N′-1,2,3-thiadiazol-5′-ylurea)
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Sanago, M.H.M., Shattuck, V.I. & Strommer, J. Rapid plant regeneration of pea using thidiazuron. Plant Cell Tiss Organ Cult 45, 165–168 (1996). https://doi.org/10.1007/BF00048761
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DOI: https://doi.org/10.1007/BF00048761