Abstract
The influence of initial concentrations of two of the major medium components, sucrose and nitrate, on the growth and the production of alkaloids and anthraquinones in cell suspension cultures of Cinchona ledgeriana Moens was studied. It was found that maximum growth and maximum alkaloid yield were obtained with a B5-medium containing the normal level of nitrate and 4% sucrose, whereas the anthraquinone yield was maximum at 8% sucrose at the normal level of nitrate. Maximum contents of secondary metabolites, expressed as μg.gDW-1, were found using a medium containing 2% sucrose, but four times the normal nitrate concentration.
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Wijnsma, R., Verpoorte, R., Harkes, P.A.A. et al. The influence of initial sucrose and nitrate concentrations on the growth of Cinchona ledgeriana cell suspension cultures and the production of alkloids and anthraquinones. Plant Cell Tiss Organ Cult 7, 21–29 (1986). https://doi.org/10.1007/BF00043917
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DOI: https://doi.org/10.1007/BF00043917