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Stability of alkaloid production in cell suspension cultures of Tabernaemontana divaricata during long-term subculture

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Abstract

Two cell lines of Tabernaemontana divaricata cell suspension culture with different growth and alkaloid production profiles were transferred to the same medium. During 30 subcultures the changes in growth and alkaloid production were followed and compared to those of the original cell lines. The presence of NAA and BAP in the medium resulted in an increase of biomass and alkaloid yield. The effect on the growth proved to be stable during these 30 subcultures. Alkaloid production showed a maximum in the 4th subculture after the change of the medium, and stabilized on a higher level than found in the original cell lines. During some growth cycles also the activities of tryptophan decarboxylase (TDC), strictosidine synthase (SSS), and phenylalanineammonia-lyase (PAL) were measured. In both the original cell lines and the derived cell lines, growth and alkaloid production proved to be stable all through the experiment, although the derived cell lines had a period of adaptation to the new medium with increased productivity.

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Abbreviations

2,4-D:

2,4-dichlorophenoxyacetic acid

NAA:

naphthaleneacetic acid

BAP:

benzylaminopurine

DW:

dry weight

TDC:

tryptophan decarboxylase

SSS:

strictosidine synthase

PAL:

phenylalanineammonia-lyase

PAT:

phenylalanineammonia-transaminase

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Authors and Affiliations

  1. Biotechnology Delft-Leiden (BDL), Project-group Plant Cell Biotechnology. Department Pharmacognosy, Center for Bio-Pharmaceutical Sciences, University of Leiden, P.O. Box 9502, 2300 RA, Leiden, The Netherlands

    Marta I. Sierra, Robert van der Heijden, Theo van der Leer & Robert Verpoorte

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  1. Marta I. Sierra
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  2. Robert van der Heijden
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  3. Theo van der Leer
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  4. Robert Verpoorte
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Sierra, M.I., van der Heijden, R., van der Leer, T. et al. Stability of alkaloid production in cell suspension cultures of Tabernaemontana divaricata during long-term subculture. Plant Cell Tiss Organ Cult 28, 59–68 (1992). https://doi.org/10.1007/BF00039916

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  • DOI: https://doi.org/10.1007/BF00039916

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