Abstract
We found an RNA editing site in the protein coding region of tobacco (Nicotiana tabacum) petB transcripts. This editing (CCA to CUA) leads to an amino acid conversion from proline to leucine. It is observed not only in chloroplasts isolated from tobacco leaves but also in non-photosynthetic proplastids isolated from a tobacco cell culture. Also unspliced pre-mRNA shows complete editing. The editing site is the same as that recently observed in the maize petB transcripts which restores the codon for a highly conserved leucine residue, suggesting that RNA editing at this site is critical for the protein (cytochrome b6) function.
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Hirose, T., Wakasugi, T., Sugiura, M. et al. RNA editing of tobacco petB mRNAs occurs both in chloroplasts and non-photosynthetic proplastids. Plant Mol Biol 26, 509–513 (1994). https://doi.org/10.1007/BF00039562
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DOI: https://doi.org/10.1007/BF00039562