Abstract
Genes encoding the phycobilisome core subunits allophycocyanin α and β and a small core linker protein in Synechocystis sp. strain PCC 6714 were cloned and sequenced. These genes form an operon, apcABC, with a single transcription start site and two possible termination sites, one following apcB and the other following apcC. The promoter region, like those of the apcABC operons of other cyanobacteria, does not resemble the consensus promoter sequences of Escherichia coli. However, the apcABC promoters identified in four strains of cyanobacteria have conserved sequences centered at −50 and −10 with respect to the start of transcription. The apcE gene, encoding the protein that links the phycobilisome core to the thylakoid membrane, was also cloned from Synechocystis 6714 and sequenced. It is unlinked to the apcABC operon. As in other Synechocystis strains, the LCM polypeptide encoded by the apcE gene contains three repeats of the basic phycobiliprotein linker domain. The apcE gene promoter sequence bears little resemblance to either the E. coli consensus or the apcABC promoter region, but it is similar to the corresponding regions of other cyanobacterial apcE genes. In these cases, there are conserved sequences centered at −40 and −10 with respect to the transcription start site. These conserved promoter elements from the apcABC and apcE genes were also identified in the corresponding 5′-flanking regions of eleven transcript starts for cpc genes encoding phycocyanin subunits in cyanobacteria and algal chloroplasts. These results suggest that a factor yet to be described participates in transcription of phycobiliprotein genes.
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References
Anderson LK, Grossman AR: Genes for phycocyanin subunits in Synechocystis sp. strain PCC-6701 and assembly mutant UV16. J Bact 172: 1289–1296 (1990).
Astier C, Elmorjani K, Meyer I, Joset F, Herdman M: Photosynthetic mutants of the cyanobacterium Synechocystis sp. strains PCC 6714 and PCC 6803: sodium p-hydroxymercuribenzoate as a selective agent. J Bact 158: 659–664 (1984).
Belknap WR, Haselkorn R: Cloning and light regulation of expression of the phycocyanin operon of the cyanobacterium Anabaena. EMBO J 6: 871–884 (1987).
Brusslan J: The psbA multigene family of Synechococcus sp. PCC 7942. Ph.D. dissertation, University of Chicago (1988).
Bryant DA: The cyanobacterial photosynthetic apparatus: comparison to those of higher plants and photosynthetic bacteria. In: Platt T, Li WKW (eds) Photosynthetic Picoplankton, pp. 423–500. Department of Fisheries and Oceans, Canadian Bulletin of Fisheries and Aquatic Sciences (1987).
Bryant DA: Genetic analysis of phycobilisome biosynthesis, assembly, structure, and function in the cyanobacterium Synechococcus sp PCC 7002. In: Stevens SEJr, Bryant DA (eds) Light-Energy Transduction in Photosynthesis: Higher Plants and Bacterial Models, pp. 62–90. American Society of Plant Physiologists, Rockville (1988).
Bryant DA: Cyanobacterial phycobilisomes: progress towards a complete structural and functional analysis via molecular genetics. In: Bogorad L, Vasil IK (eds) The Photosynthetic Apparatus: Molecular Biology and Operation, pp. 257–300. Academic Press, New York (1991).
Bryant DA, deLorimier R, Lambert DH, Dubbs JM, Stirewalt VL, Stevens SEJr., Porter RD, Tam J, Jay E: Molecular cloning and nucleotide sequence of the alpha and beta subunits of allophycocyanin from the cyanelle genome of Cyanophora paradoxa. Proc Natl Acad Sci USA 82: 3242–3246 (1985).
Bryant DA, Glazer AN, Eiserling FA: Characterization and structural properties of the major biliproteins of Anabaena sp. Arch Microbiol 110: 61–75 (1976).
Bryant DA, Guglielmi G, Tandeau de Marsac N, Houmard A-M, Cohen-Bazire G: The structure of cyanobacterial phycobilisomes: a model. Arch Microbiol 123: 113–127 (1979).
Capuano V, Braux A, Tandeau de Marsac N, Houmard J: The ‘anchor polypeptide’ of cyanobacterial phycobilisomes. Molecular characterization of the Synechococcus sp. PCC 6301 apcE gene. J Biol Chem 266: 7239–7247 (1991).
Conley PB, Lemaux PG, Grossman A: Molecular characterization and evolution of sequences encoding light-harvesting components in the chromatically adapting cyanobacterium Fremyella diplosiphon. J Mol Biol 199: 447–465 (1988).
Glazer AN: Light guide: directional energy transfer in a photosynthetic antenna. J Biol Chem 264: 1–4 (1989).
Glazer AN, Lundell DJ, Yamanaka G, Williams RC: The structure of a ‘simple’ phycobilisome. Ann Microbiol (Inst Pasteur) 134B: 159–180 (1983).
Golden SS, Brusslan J, Haselkorn R: Expression of a family of psbA genes encoding a photosystem II polypeptide in the cyanobacterium Anacystis nidulans R2. EMBO J 5: 2789–2798 (1986).
Golden SS, Brusslan J, Haselkorn R: Genetic engineering of the cyanobacterial chromosome. Meth Enzymol 153: 215–231 (1987).
Herdman M, Delaney SF, Carr NG: A new medium for the isolation and growth of auxotrophic mutants of the blue green alga Anacystis nidulans. J Gen Microbiol 79: 233–237 (1973).
Houmard J, Capuano V, Colombano MV, Coursin T, Tandeau de Marsac N: Molecular characterization of the terminal energy acceptor of cyanobacterial phycobilisomes. Proc Natl Acad Sci USA 87: 2152–2156 (1990).
Houmard J, Mazel D, Mogeut C, Bryant DA, Tandeau de Marsac N: Organization and nucleotide sequence of genes encoding core components of the phycobilisomes from Synechococcus 6301. Mol Gen Genet 205: 404–410 (1986).
Kalla SR, Lind LK, Lidholm J, Gustafsson P: Transcriptional organization of the phycocyanin subunit gene clusters of the cyanobacterium Anacystis nidulans UTEX 625 J Bact 170: 2961–2970 (1988).
Kraft RJ, Tardiff J, Krauter KS, Leinwand LA: Using mini-prep plasmid DNA for sequencing double stranded templates with Sequenase. BioTechniques 6: 544–547 (1988).
Lemaux PG, Grossman AR: Major light-harvesting polypeptides encoded in polycistronic transcripts in a eukaryotic alga. EMBO J 4: 1911–1919 (1985).
Lundell DJ, Glazer AN: Molecular architecture of a light-harvesting antenna-quaternary interactions in the Synechococcus 6301 phycobilisome core as revealed by partial tryptic digestion and circular dichroism studies. J Biol Chem 258: 8708–8713 (1983).
Maniatis T, Fritsch EF, Sambrook J: Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (1982).
Pilot TJ, Fox JL: Cloning and sequencing of the genes encoding the alpha and beta subunits of C-phycocyanin from the cyanobacterium Agmenellum quadruplicatum. Proc Natl Acad Sci USA 81: 6983–6987 (1984).
Platt T: Termination of transcription and its regulation in the tryptophan operon of E. coli. Cell 24: 10–23 (1981).
Rosenberg M, Court D: Regulatory sequences involved in the promotion and termination of RNA transcription. Annu Rev Genet 13: 319–353 (1979).
Schneider GJ, Lang JD, Haselkorn R: Promoter recognition by the RNA polymerase from vegetative cells of the cyanobacterium Anabaena 7120. Gene 105: 51–60 (1991).
Schneider GJ, Tumer NE, Richaud C, Borbely G, Haselkorn R: Purification and characterization of RNA polymerase from the cyanobacterium Anabaena 7120. J Biol Chem 262: 14633–14639 (1987).
Tandeau de Marsac N, Cohen-Bazire G: Molecular composition of cyanobacterial phycobilisomes. Proc Natl Acad Sci USA 74: 1635–1639 (1977).
Yankovsky NK, Fonstein MY, Lashina SY, Bukanov NO, Yakubovich NV, Ermakova LM, Rebentish BA, Janulaitis AA, Debabov VG: Phasmids as effective and simple tools for construction and analysis of gene libraries. Gene 81: 203–210 (1989).
Zilinskas BA, Howell DA: Comparative immunology of the phycobilisome lknker polypeptides. In: Progress in Photosynthesis Research vol 2, pp. 161–164 (1987).
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DiMagno, L., Haselkorn, R. Isolation and characterization of the genes encoding allophycocyanin subunits and two linker proteins from Synechocystis 6714. Plant Mol Biol 21, 835–845 (1993). https://doi.org/10.1007/BF00027115
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DOI: https://doi.org/10.1007/BF00027115