Abstract
Nutrient limitation of periphyton and phytoplankton was assessed in the Upper Guadalupe River, Texas USA. Nutrient-diffusing substrates with added nitrogen (N) and phosphorus (P) were used to identify the limiting nutrient for lotic algae at three river sites in summer, fall, and winter. Pots enriched with P had significantly higher chlorophyll a concentrations for 7 of 9 trials. Added N alone did not significantly increase algal standing crops, although it was found to be secondarily limiting on one (and possibly two) occasions. Flow-through enrichment experiments were conducted in order to quantify the concentration of P needed to significantly increase algal standing crops. Response to enrichment was rapid when ambient P concentration was low (< 0.010 mg L−1), but more moderate when ambient P levels were higher (0.015–0.025 mg L−1). Nutrient limitation of phytoplankton in small surface-release reservoirs varied throughout the study, but N was either primarily or secondarily limiting in 6 of 8 trials; shifts in the limiting nutrient were correlated with fluctuations in flow into the reservoirs. Our enrichment studies show that algal response to nutrient addition was unpredictable as phytoplankton tended to be N-limited while periphyton was mainly P-limited. Further, while discharge apparently dictated the nutrient-biomass relationship for phytoplankton in reservoirs, ambient nutrient level is an important determinant of lotic periphyton response to enrichment.
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References
Bothwell M. L., 1988. Growth rate responses of lotic periphytic diatoms to experimental phosphorus enrichment: the influence of temperature and light. Can. J. Fish. aquat. Sci. 45: 261–270.
Bothwell, M. L., 1989. Phosphorus limited growth dynamics of lotic periphytic diatom communities: areal biomass and cellular growth rate responses. Can. J. Fish. aquat. Sci. 46: 1293–1301.
Bott, T. L., 1983. Primary productivity in streams. In G. W. Minshall and J. R. Barnes (eds.), Stream ecology: application and testing of general ecological theory. Plenum Press, New York. pp. 29–53.
Coler, R. A. & L. Swift, 1980. Demonstrating the limiting nutrient content in rural river reaches. Am. Biol. Teach. 42: 353–355.
Edson, J. J. & R. C. Jones, 1988. Spatial, temporal, and stream runoff-related variations in phytoplankton community structure in a small suburban reservoir. Hydrobiologia 169: 353–362.
Elser, J. J. & B. L. Kimmel, 1985. Nutrient availability for phytoplankton production in a multiple-impoundment series. Can. J. Fish. aquat. Sci. 42: 1359–1370.
Elser, J. J., M. M. Elser, N. A. MacKay & S. R. Carpenter, 1980. Zooplankton-mediated transitions between N and P limited algal growth. Limnol. Oceanogr. 33: 1–14.
Elwood, J. W., J. D. Newbold, A. F. Trimble & R. W. Stark, 1981. The limiting role of phosphorus in a woodland stream ecosystem: effects of P enrichment on leaf decomposition and primary producers. Ecology 62: 146–158.
Fairchild, G. W. & R. L. Lowe, 1984. Artificial substrates which release nutrients: effects on periphyton and invertebrate succession. Hydrobiologia 114: 29–37.
Fairchild, G. W., R. L. Lowe & W. B. Richardson, 1985. Algal periphyton growth on nutrient-diffusing substrates: an in situ bioassay. Ecology 66: 465–472.
Goldman, C. R., 1962. A method of studying nutrient limiting factors in situ in water columns isolated by polyethylene film. Limnol. Oceanogr. 7: 99–101.
Grimm, N. B. & S. G. Fisher, 1986. Nitrogen limitation in a Sonoran desert stream. J. N. Am. Benth. Soc. 5: 2–15.
Hannan, H. H., 1979. Chemical modifications in reservoir-regulated streams. In J. V. Ward & J. A. Stanford (eds.), The ecology of regulated streams. Plenum Press, New York, pp. 75–94.
Hannan, H. H., D. B. Barrows, I. R. Fuchs, R. A. Segura & D. C. Whitenburg, 1980. Limnological and operational factors affecting water quality in Canyon Reservoir, Texas. In E. E. Driver & W. O. Wunderlich (eds.), Environmental effects of hydraulic engineering works. Tennessee Valley Authority, Norris, TN. pp. 39–48.
Hannan, H. H., W. C. Young & J. J. Mayhew, 1973. Nitrogen and phosphorus in a stretch of the Guadalupe River, Texas, with five main-stream impoundments. Hydrobiologia 43: 419–441.
Hecky, R. E. & P. Kilham, 1988. Nutrient limitation of phytoplankton in freshwater and marine environments: A review of recent evidence on the effects of enrichment. Limnol. Oceanogr. 33: 796–822.
Komarkova, J., 1974. Limitation of phytoplankton growth by lack of nutrients in two reservoirs in Czechoslovakia. Arch. Hydrobiologia/Suppl. 46- Algological Studies 10: 55–89.
Likens, G. E., 1972. Nutrients and eutrophication: the limiting nutrient controversy. Proceedings of the symposium on nutrients and eutrophication. American Society of Limnology and Oceanography, W. K. Kellogg Biological Station, Michigan.
Mason, C. F., 1981. Biology of freshwater pollution. Longman, London.
McCracken, M. D., 1980. Periphyton. In E. D. LeCren & R. H. Lowe-McConnell (eds.), The functioning of freshwater ecosystems. Cambridge University Press, Cambridge. pp. 212–213.
Peterson, B. J., J. E. Hobbie, T. L. Corliss & K. Kriet, 1983. A continuous-flow periphyton bioassay: tests of nutrient limitation in a tundra stream. Limnol. Oceanogr. 28: 583–590.
Peterson, B. J., J. E. Hobbie, A. E. Hershey, M. A. Lock, T. E. Ford, J. R. Vestal, V. L. McKinley, M. A. J. Hullar, M. C. Miller, R. M. Vetullo & G. S. Volk, 1985. Transformation of a tundra river from heterotrophy to autotrophy by addition of phosphorus. Science 229: 1383–1386.
Powers, C. F., D. W. Schultz, K. W. Malueg, R. M. Brice & M. D. Schuldt, 1972. Algal responses to nutrient additions in natural waters. II. Field experiments. In G. E. Likens (ed.), Nutrients and eutrophication: the limiting nutrient controversy. Proceedings of the symposium on nutrients and eutrophication. American Society of limnology and Oceanography, W. K. Kellogg Biological Station, Michigan. pp. 141–154.
Pringle, C. M. & J. A. Bowers, 1984. An in situ substratum fertilization technique: diatom colonization on nutrient-enriched, sand substrata. Can. J. Fish. aquat. Sci. 41: 1247–1251.
Rhee, G-Y, 1980. Optimal N: P ratios and coexistence of planktonic algae. J. Phycol. 16: 486–489.
Riber, H. H. & R. G. Wetzel, 1987. Boundary-layer and internal diffusion effects on phosphorus fluxes in lake periphyton. Limnol. Oceanogr. 32: 1181–1194.
Shanz, F. & J. Juon, 1983. Two different methods of evaluating nutrient limitations of periphyton bioassays, using water from the River Rhine and eight of its tributaries. Hydrobiologia 102: 187–195.
Schindler, D. W., F. A. J. Armstrong, S. K. Holmgren & G. J. Brunskill, 1971. Eutrophication of Lake 227, Experimental Lakes Area, northwestern Ontario, by addition of phosphate and nitrate. J. Fish. Res. Bd Can. 28: 1763–1782.
Søballe, D. M. & B. L. Kimmel, 1987. A large-scale comparison of factors influencing phytoplankton abundance in rivers, lakes, and impoundments. Ecology 68: 1943–1954.
Stockner, J. A. & K. R. S. Shortreed, 1978. Enhancement of autotrophic production by nutrient addition in a coastal rainforest stream on Vancouver Island. J. Fish. Res. Bd Can. 35: 28–34.
Strahler, A. N., 1957. Quantitative analysis of watershed geomorphology. Trans. Am. Geophys. Union 38: 913–920.
Taylor, M. P. & E. B. Welch, 1971. Norris Reservoir fertilizer study II. Effects of thermal stratification and nutrient availability on the productivity of reservoir phytoplankton. J. Tenn. Acad. Sci. 46: 90–97.
Triska, F. J., V. C. Kennedy, R. J. Avanzino & B. N. Reilly, 1983. Effects of simulated canopy cover on regulation of nitrate uptake and primary production by natural periphyton assemblages. In T. D. Fontaine and S. M. Bartell (eds.), Dynamics of lotic ecosystems. Ann Arbor Science Publishers, Ann Arbor, Michigan. pp. 129–160.
Welch, E. B., 1984. Lake restoration results. In F. B. Taub (ed.), Lakes and reservoirs. Ecosystems of the world 23. Elsevier, Amsterdam. pp. 557–571.
Wetzel, R. G. & G. E. Likens. Limnological analyses. W. B. Saunders, Philadelphia.
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Stanley, E.H., Short, R.A., Harrison, J.W. et al. Variation in nutrient limitation of lotic and lentic algal communities in a Texas (USA) river. Hydrobiologia 206, 61–71 (1990). https://doi.org/10.1007/BF00018970
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DOI: https://doi.org/10.1007/BF00018970