Abstract
Ciguatoxin, the principal toxin of ciguatera produced by a toxic dinoflagellate and accumulated through the food chain in the viscera of moray eels, was purified. The probable molecular formula C60H86O19 was obtained for the first time by high resolution mass spectrometry. 1H NMR spectra suggested the presence of a primary hydroxyl group suitable for preparing an antigen necessary to develop an enzyme immunoassay.
Similar content being viewed by others
References
Adachi R, Fukuyo Y (1978) The thecal structure of a marine toxic dinoflagellate Gambierdiscus toxicus gen. et sp. nov. collected in a ciguatera-endemic area. Nippon Suisan Gakkaishi 45: 67–71.
Bagnis R, Chanteau S, Yasumoto T (1977) Un agent étiologique vraisemblable de la ciguatera. C. R. Acad. Sc. Paris, t. 285 (4 juillet 1977). Serie D: 105–107.
Bagnis R, Kuberski T, Laugier R (1979) Clinical observations on 3,009 cases of ciguatera (fish poisoning) in the South Pacific. Am. J. Trop. Med. Hyg. 28: 1067–1073.
Boyarsky LL, Rayner ME (1970) The effect of ciguatera toxin on Aplysia neurons. Proc. Soc. exp. biol. Med. 134: 332–335.
Chungue E, Bagnis R, Fusetani N, Yasumoto T (1977) Le complexe toxinique des poissons peroquets. Biochimie 59: 739–741.
Halstead BW (1965) Poisonous and Venomous Marine Animals of the World, 1. U.S. Gov. Print. Office, Washington, D.C., 994 pp.
Joh YG, Scheuer PJ (1986) The chemical nature of scaritoxin. Mar. fish. Review 48: 19–22.
Meier J, Theakston RDG (1986) Approximate LD50 determination of snake venoms using eight to ten experimental animals. Toxicon 24: 395–401.
Nukina M, Koyanagi LM, Scheuer PJ (1984) Two interchangeable forms of ciguatoxin. Toxicon 22: 169–176.
Quod JP, Legrand AM (1987) Effects of partially purified ciguatoxin from moray eel Gymnothorax javanicus on action potential of isolated rat heart. In Gopalakrishnakone P, Tan CK (eds), Progress in Venom and Toxin Research. Faculty of Med., National University of Singapore, Singapore, 394–404.
Scheuer PJ, Takahashi W, Tsutsumi J, Yoshida T (1967) Ciguatoxin: isolation and chemical nature. Science 155: 1267–1268.
Seino A, Kobayashi M, Momose K, Yasumoto T, Ohizumi Y (1988) The mode of inotropic action of ciguatoxin on guinea-pig cardiac muscle. Br. J. Pharmacol. 95: 876–882.
Tachibana K (1980) Structural studies on marine toxins. Ph.D. Thesis, University of Hawaii, Honolulu.
Tachibana K, Nukina M, Joh YG, Scheuer PJ (1987) Recent developments in the molecular structure of ciguatoxin. Biol. Bull. 172: 122–127.
Yasumoto T, Scheuer PJ (1969) Marine toxins of the Pacific VIII ciguatoxin from moray eel livers. Toxicon 7: 273–276.
Yasumoto T, Hashimoto, Bagnis R, Randall JE, Banner AH (1971) Toxicities of the surgeonfishes. Nippon Suisan Gakkaishi 31: 724–734.
Yasumoto T, Nakajima I, Bagnis R, Adachi R (1977) Finding of a dinoflagellate as a likely culprit of ciguatera. Nippon Suisan Gakkaishi 43: 1021–1026.
Author information
Authors and Affiliations
Additional information
author for correspondence
Rights and permissions
About this article
Cite this article
Legrand, A.M., Litaudon, M., Genthon, J.N. et al. Isolation and some properties of ciguatoxin. J Appl Phycol 1, 183–188 (1989). https://doi.org/10.1007/BF00003882
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00003882