Skip to main content
SpringerLink
Log in

Analysis of Zearalenone in Wheat Using High-Performance Liquid Chromatography with Fluorescence Detection and/or Enzyme-Linked Immunosorbent Assay

  • Pathology
  • Published:
Cereal Research Communications Aims and scope Submit manuscript

Summary

In the first phase of experiments, performance characteristics of two alternative analytical methods used for the determination of zearalenone and its metabolites were assessed in presented study. Extraction of ground grains with acetonitrile-water (84/16, v/v) mixture followed by clean-up using GPC BioBeads S-X3 soft gel were employed prior to HPLC (reversed phase column C18). The use of ELISA method allowed direct, highly sensitive measurement of zearalenone levels in crude extracts. For ELISA method, extraction with methanol-water (70/30, v/v) without any clean-up was used. Compared to HPLC/FLD method, the precision of ELISA method was inferior.

The second part of study was concerned with toxic potential of two isolates of Fusarium culmorum (W. G. Sm.) Sacc. under field conditions. Analyses of wheat varieties tested for the content of zearalenone were carried out. Levels of zearalenone in wheat inoculated by more toxic isolate (B) ranged from 309.1 to 2162.7 µg/kg. These values were higher almost by 2 orders of magnitude compared to levels found in samples inoculated with isolate A. Wheat variety SPARTA was identified as the most resistant against fungal attack from all checked varieties.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Long, G.G., Diekman, M., Tuite, J.F., Shannon, G.M., Vesonder, R.F., Amer. Vet. Res., 43 (1982) 1599

    CAS  Google Scholar 

  2. Warner, R., Ram, B. P., Hart L. P., and Pestka J., J. Agric. Food Chem. 34 (1986) 714

    Article  CAS  Google Scholar 

  3. Dorner, J. W., Mycotoxins in Food: Methods of Analysis, in Handbook of Food Analysis (1997) Hogeschool Gent, Belgium

    Google Scholar 

  4. Richard, J. L., Bennett, G. A., Ross, P. F., Nelson, P. E., J. Anim. Sci. 71 (1993) 2563

    Article  CAS  Google Scholar 

  5. Krska, R., Schuhmacher, R., Grasserbauer, H., Cer. Res. Comm., 25 (1997) 327–329

    CAS  Google Scholar 

  6. Wolf-Hall, Ch. E., Bullerman, L. B., J. of Food Prot., 59 (1996) 438–440

    Article  CAS  Google Scholar 

  7. Langseth, W., Rundberget, T., J. Chromatog. A., 815 (1998) 103–121

    Article  CAS  Google Scholar 

  8. Tacke, B. K., Casper, H. H., J. of AOAC Int., 79 (1996) 472–475

    CAS  Google Scholar 

  9. Weingaertner, J., Krska R., Fresenius J. Anal. Chem., 357 (1997) 1206–1210

    Article  CAS  Google Scholar 

  10. Šip, V., Stuchlíková, E., Czech J. Genet. Plant. Breed., 36 (2000) 49–58

    Google Scholar 

  11. Mesterházy, Á., Plant Breeding 98 (1987) 25

    Article  Google Scholar 

  12. Radová, Z., Holadová, K., Hajšlová, J., J. Chromatog. A, 829 (1998) 259

    Article  Google Scholar 

  13. Smith, J. E., Lewis C. V., Anderson J. G., Solomons, G. L., Mycotoxins in Human Nutrition and Health (1994) UER 16048 EN Agroindustrial Research Division

  14. D’Mello, P. F., Duffus, C. M., Duffus, J. H., Mycotoxins. In Toxic Substances in Crop Plant (1991) Royal Society of Chemistry, Cambridge

    Chapter  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. The Technical Institute of Chemistry Prague, Technická 3, 166 28, Prague, Czech Republic

    Zuzana Radová, Jana Hajšlová & Jana Králová

  2. Research Institute of Crop Production, Drnovská 507, 161 06, Prague 6, Czech Republic

    Ludmila Papoušková & Světlana Sýkorová

Authors
  1. Zuzana Radová
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jana Hajšlová
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jana Králová
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ludmila Papoušková
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Světlana Sýkorová
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jana Hajšlová.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Radová, Z., Hajšlová, J., Králová, J. et al. Analysis of Zearalenone in Wheat Using High-Performance Liquid Chromatography with Fluorescence Detection and/or Enzyme-Linked Immunosorbent Assay. CEREAL RESEARCH COMMUNICATIONS 29, 435–442 (2001). https://doi.org/10.1007/BF03543692

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF03543692

Keywords

Search

Navigation