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Toxicity, of extracts of barley kernels inoculated withFusarium spp. toArtemia salina

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Abstract

Toxicity toA. salina, of the Fusarium metabolites: deoxynivalenol (DON), its acetylated derivatives (3- and 15-AcDON), zearalenone (ZON), neosolaniol (NEO), nivalenol (NIV), T-2, HT-2 toxins, has been examined and compared with toxicity of extracts of barley kernels (8 cultivars and 4 lines) inoculated withFusarium culmorum, F. graminearum andF. sporotrichioides respectively. Estimated LC50 values were expressed as relative toxicity (RT) in mg DON/kg for samples inoculated withF. culmorum, F. graminearum or in mg T-2/kg forF. sporotrichioides inoculations.

Toxicity of extracts of the same genotype/line kernels was compared among different pathogens used for inoculation and differences in Fusarium head blight susceptibility of different genotypes/lines inoculated with the sameFusarium strain were found. Significant correlation between toxicity of extracts (LC50, RT) and toxic metabolites concentration was found (\(\bar r = 0.82\); P = 0.01).

Bioassays withA. Salina offer a fast, easy and inexpensive method to examine cereal genotypes susceptibility to Fusarium head blight and mycotoxins accumulation in kernels.

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Foremska, E., Kiecana, I., Perkowski, J. et al. Toxicity, of extracts of barley kernels inoculated withFusarium spp. toArtemia salina . Mycotox Res 12, 67–72 (1996). https://doi.org/10.1007/BF03192264

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