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Effect of phosphorus deficiency on acid phosphatase and phytase activities in common bean (Phaseolus vulgaris L.) under symbiotic nitrogen fixation

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Abstract

Changes in growth, symbiotic nitrogen fixation (SNF), acid phosphatase (ACP), and phytase activities to phosphorus availability (15 and 60 μmol KH2PO4 plant−1 week−1) were compared in two recombinant lines (115 and 147) of common bean. Plant growth, nodulation and SNF were genotype and P level-dependent. 147 was more affected by P shortage (15 μmol P) than 115. Four ACP types were revealed in the nodules of both lines, ACP1 exhibiting a higher specific activity under P shortage as compared to the 60 μmol P treatment, especially in 115. A single phytase was revealed for the nodules of both lines and was significantly enhanced by P deficiency. Three ACP types were found in roots and leaves, showing increasing activity under P deficiency, especially in 115. Regardless of P supply, leaf ACP specific activity was higher than that of nodules and roots in the both lines. Interestingly, phosphorus use efficiency for N2 fixation significantly correlated to nodule ACP activity under P shortage in the both lines. The relatively better performance of 115 as compared to 147 under P deficiency could be partly ascribed to the ability of 115 to maintain higher ACP activity. This enzyme might be involved in the remobilization of the plant Pi and its utilization for SNF.

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  1. Ahmed Debez

    Present address: Institut für Botanik, Leibniz Universität Hannover, Herrenhäuser Str. 2, 30419, Hannover, Germany

Authors and Affiliations

  1. Laboratoire d’Adaptation des Plantes aux Stress Abiotiques, Centre de Biotechnologie à la Technopole de Borj Cedria, BP 901, 2050, Hammam-Lif, Tunisia

    Saber Kouas, Ahmed Debez & Chedly Abdelly

  2. Rhizosphere et Symbiose, Institut National de la Recherche Agronomique (UMR 1220), 1 place Viala, 34060, Montpellier, France

    Julien Louche, Claude Plassard & Jean Jacques Drevon

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  1. Saber Kouas
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Correspondence to Chedly Abdelly.

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Kouas, S., Louche, J., Debez, A. et al. Effect of phosphorus deficiency on acid phosphatase and phytase activities in common bean (Phaseolus vulgaris L.) under symbiotic nitrogen fixation. Symbiosis 47, 141–149 (2009). https://doi.org/10.1007/BF03179974

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