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A novel alkaline and low-temperature lipase ofBurkholderia cepacia isolated from Bohai in China for detergent formulation

  • Industrial Microbiology
  • Original Articles
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Abstract

The bacterial strain LP08 was isolated from soil collected from bay of Bohai, China. The sequence of 16S rDNA of strain LP08 showed 99% homology toBurkholderia cepacia. The lipase fromBurkholderia cepacia LP08 was purified by ammonium sulphate precipitation, ion exchange chromatography and Sephadex G-75 chromatography. The characterization of the lipase exhibited maximum activity at 30 °C and pH 9.0. The lipase retained 63, 66, 74, and 95% of its maximum activity at 10, 15, 20 and 25 °C respectively. The lipase activity was promoted in the presence of commercial detergent, sodium cholate, sodium taurocholate, glycerine and NaCl, while was little inhibited in the presence of Triton X-100, Tween-20, Tween-80, SDS, saponin. The present lipase was highly stable towards oxidizing agents and was stable after 1 h at 25 °C in the presence of hydrogen peroxide, sodium hypochlorite and sodium perborate. The results suggest that the lipase fromBurkholderia cepacia LP08 showed good potential for application in the detergent formulation.

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Authors and Affiliations

  1. Key Laboratory of Industrial Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, PO: 300457, Tianjin, P.R. China

    HaiKuan Wang, RuiJuan Liu, FuPing Lu, Wei Qi, Jing Shao & HuiJing Ma

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  1. HaiKuan Wang
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  2. RuiJuan Liu
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  3. FuPing Lu
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  4. Wei Qi
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  5. Jing Shao
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  6. HuiJing Ma
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Correspondence to HaiKuan Wang or FuPing Lu.

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Wang, H., Liu, R., Lu, F. et al. A novel alkaline and low-temperature lipase ofBurkholderia cepacia isolated from Bohai in China for detergent formulation. Ann. Microbiol. 59, 105–110 (2009). https://doi.org/10.1007/BF03175606

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  • DOI: https://doi.org/10.1007/BF03175606

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