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Identification of yeast population dynamics of spontaneous fermentation in Beijing wine region, China

  • Food Microbiology
  • Original Articles
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Abstract

The aim of this study was (i) to investigate changes occurring in the yeast population profile during spontaneous fermentation of grape juice; (ii) to assess the proliferation of commercial yeast starter culture strains in vineyards; and (iii) to identify indigenous wine strains for future development of starter strains that better reflect the yeast biodiversity of China’s grape-growing regions. To achieve this, yeasts were isolated at four different stages during fermentation of both hand-pressed and winery-sourced must samples ofVitis vinifera L. cv. Roussanne and Merlot. A total of 1600 yeast colonies were isolated and then grouped according to macroscopic and microscopic characteristics. A selection of 291 colonies from the different groups was subjected to species identification using the internal transcribed spacer regions of the 5.8S rRNA gene (ITS1-5.8S-ITS2 region) and the inter-delta (δ) sequence of the 26S rRNA D1/D2 region. In addition, 104Saccharomyces cerevisiae colonies were subjected to strain identification. Twelve species belonging to nine different genera were found amongst the isolates. During the early stages of fermentation, it was found thatHanseniaspora uvarum andCandida stellata numerically dominated the four to six yeast species present, including a region-specific yeast,Sporobolomyces beijingensis. TwoS. cerevisiae strains were isolated from the final stage of fermentation. These two indigenous strains, which were found to be different from the nine commercial yeast strains previously used as starter cultures in this particular Beijing-based winery, might possess potentially important region-specific oenological characteristics. This study provides the first essential step towards the preservation and exploitation of the hidden oenological potential of the untapped wealth of yeast biodiversity in China’s wine-producing regions.

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References

  • Agnolucci M., Scarano S., Santoro S., Sassano C., Toffanin A., Nuti M. (2007). Genetic and phenotypic diversity of autochthonousSaccharomyces spp. strains associated to natural fermentation of ‘Malvasia delle Lipari’. Lett. Appl. Microbiol., 45: 657–662.

    Article  CAS  PubMed  Google Scholar 

  • Beltran G., Torija M. J., Novo M., Ferrer N., Poblet M., Guillamon J.M., Rozes N., Mas A. (2002). Analysis of yeast populations during alcoholic fermentation: a six year follow-up study. Syst. Appl. Microbiol., 25: 287–293.

    Article  CAS  PubMed  Google Scholar 

  • Combina M., Elia A., Mercado L., Catania C., Ganga A., Martinez C. (2005). Dynamics of indigenous yeast populations during spontaneous fermentation of wines from Mendoza, Argentina. Int. J. Food Microbiol., 99: 237–243.

    Article  CAS  PubMed  Google Scholar 

  • Constanti M., Poblet M., Arola L., Mas A., Guillamón J.M. (1997). Analysis of yeast populations during alcoholic fermentation in a newly established winery. Am. J. Enol. Vitic. 48: 339–344.

    Google Scholar 

  • De La Torre M.J., Millan M.C., Perez-Juan P., Morales J., Ortega J.M. (1999). Indigenous yeasts associated with twoVitis vinifera grape varieties cultured in southern Spain. Microbios, 100: 27–40.

    Google Scholar 

  • Delneri D., Colson I., Grammenoudi S., Roberts I.N., Louis E.J., Oliver S.G. (2003). Engineering evolution to study speciation in yeasts. Nature, 422: 68–72.

    Article  CAS  PubMed  Google Scholar 

  • Demuyter C., Lollier M., Legras J.L., Le Jeune C. (2004). Predominance ofSaccharomyces uvarum during spontaneous alcoholic fermentation, for three consecutive years, in an Alsatian winery. J. Appl. Microbiol., 97: 1140–1148.

    Article  CAS  PubMed  Google Scholar 

  • Di Maro E., Ercolini D., Coppola S. (2007). Yeast dynamics during spontaneous wine fermentation of the Catalanesca grape. Int. J. Food Microbiol., 117: 201–210.

    Article  PubMed  Google Scholar 

  • Domizio P., Lencioni L., Ciani M., Di Blasi S., Pontemolesi C., Sabatelli M.P. (2007). Spontaneous and inoculated yeast populations dynamics and their effect on organoleptic characters of Vinsanto wine under different process conditions. Int. J. Food Microbiol., 115: 281–289.

    Article  CAS  PubMed  Google Scholar 

  • Egli C.M., Edinger W.D., Mitrakul C.M., Henick-Kling T. (1998). Dynamics of indigeous and inoculated yeast populations and their effect on the sensory character of Riesling and Chardonnay wines. J. Appl. Microbiol., 85: 779–789.

    Article  CAS  PubMed  Google Scholar 

  • Esteve-Zarzoso B., Peris-Toran M., Garcia-Maiquez E., Uruburu F., Querol A. (2001). Yeast population dynamics during the fermentation and biological aging of sherry wines. Appl. Environ. Microbiol., 67: 2056–2061.

    Article  CAS  PubMed  Google Scholar 

  • Fell J.W., Boekhout T., Fonseca A., Scorzetti G., Statzell-Tallman A. (2000). Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis. Int. J. Syst. Evol. Microbiol., 50 Pt 3: 1351–1371.

    CAS  PubMed  Google Scholar 

  • Fleet G.H. (2003). Yeast interactions and wine flavour. Int. J. Food Microbiol., 86: 11–22.

    Article  CAS  PubMed  Google Scholar 

  • Fleet G.H., Lafon-Lafourcade S., Ribereau-Gayon P. (1984). Evolution of yeasts and lactic acid bacteria during fermentation and storage of Bordeaux wines. Appl. Environ. Microbiol., 48: 1034–1038.

    PubMed  Google Scholar 

  • Guillamon J.M., Sabate J., Barrio E., Cano J., Querol A. (1998). Rapid identification of wine yeast species based on RFLP analysis of the ribosomal internal transcribed spacer (ITS) region. Arch. Microbiol., 169: 387–392.

    Article  CAS  PubMed  Google Scholar 

  • Gutiérrez A.R., Santamaría P., Epifanio S., Garijo P., López R. (1999). Ecology of spontaneous fermentation in one winery during 5 consecutive years. Lett. Appl. Microbiol., 29: 411–415.

    Article  Google Scholar 

  • Hierro N., Gonzalez A., Mas A., Guillamon J.M. (2006). Diversity and evolution of non-Saccharomyces yeast populations during wine fermentation: effect of grape ripeness and cold maceration. FEMS Yeast Res., 6: 102–111.

    Article  CAS  PubMed  Google Scholar 

  • Jolly N.P., Augustyn O.P.H., Pretorius I.S. (2003a). The occurrence of non-Saccharomyces yeast species over three vintages on four vineyards and grape musts from four production regions of the Western Cape, South Africa. S. Afr. J. Enol. Vitic., 24: 35–42.

    Google Scholar 

  • Jolly N.P., Augustyn O.P.H., Pretorius I.S. (2003b). The effect of non-Saccharomyces yeasts on fermentation and wine quality. S. Afr. J. Enol. Vitic., 24: 55–62.

    CAS  Google Scholar 

  • Jolly N.P., Augustyn O.P.H., Pretorius I.S. (2003c). The use ofCandida pulcherrima in combination withSaccharomyces cerevisiae for the production of Chenin blanc wine. S. Afr. J. Enol. Vitic., 24: 63–69.

    CAS  Google Scholar 

  • Jolly N.P., Augustyn O.P.H., Pretorius I.S. (2006). The role and use of non-Saccharomyces yeasts in wine production. S. Afr. J. Enol. Vitic., 27: 15–39.

    CAS  Google Scholar 

  • Khan W., van der Westhuizen T.J., Augustyn O.P.H., Pretorius I.S. (2000). Geographic distribution and evaluation ofSaccharomyces cerevisiae strains isolated from vineyards in the warm, inland regions of the Western Cape in South Africa. S. Afr. J. Enol. Vitic., 21: 17–31.

    CAS  Google Scholar 

  • Lambrechts M.G., Pretorius I.S. (2000). Yeast and its importance to wine aroma — a review. S. Afr. J. Enol. Vitic., 21: 97–129.

    CAS  Google Scholar 

  • Legras J.L., Karst F. (2003). Optimisation of interdelta analysis forSaccharomyces cerevisiae strain characterisation. FEMS Microbiol. Lett, 221: 249–255.

    Article  CAS  PubMed  Google Scholar 

  • Longo E., Cansado J., Agrelo D., Villa T.G. (1991). effect of climatic conditions on yeast diversity in grape musts from Northwest Spain. Am. J. Enol. Vitic., 42: 141–144.

    Google Scholar 

  • Lopes C.A., van Broock M., Querol A., Caballero A.C. (2002).Saccharomyces cerevisiae wine yeast populations in a cold region in Argentinean Patagonia. A study at different fermentation scales. J. Appl. Microbiol., 93: 608–615.

    Article  CAS  PubMed  Google Scholar 

  • Makimura K., Murayama S. Y., Yamaguchi H. (1994). Detection of a wide range of medically important fungi by the polymerase chain reaction. J. Med. Microbiol., 40: 358–364.

    Article  CAS  PubMed  Google Scholar 

  • Mannazzu I., Simonetti E., Marinangeli P., Guerra E., Budroni M., Thangavelu M., Clementi F. (2002). SED1 gene length and sequence polymorphisms in feral strains ofSaccharomyces cerevisiae. Appl. Environ. Microbiol., 68: 5437–5444.

    Article  CAS  PubMed  Google Scholar 

  • Martinez C., Cosgaya P., Vasquez C., Gac S., Ganga A. (2007). High degree of correlation between molecular polymorphism and geographic origin of wine yeast strains. J. Appl. Microbiol., 103:2185–2195.

    Article  CAS  PubMed  Google Scholar 

  • Masneuf-Pomarede I., Le Jeune C., Durrens P., Lollier M., Aigle M., Dubourdieu D. (2007). Molecular typing of wine yeast strainsSaccharomyces bayanus var.uvarum using microsatellite markers. Syst. Appl. Microbiol., 30: 75–82.

    Article  CAS  PubMed  Google Scholar 

  • Masoud W., Cesar L.B., Jespersen L., Jakobsen M. (2004). Yeast involved in fermentation of Coffea arabica in East Africa determined by genotyping and by direct denaturating gradient gel electrophoresis. Yeast, 21: 549–556.

    Article  CAS  PubMed  Google Scholar 

  • Mills D.A., Johannsen E.A., Cocolin L. (2002). Yeast diversity and persistence in botrytis-affected wine fermentations. Appl. Environ. Microbiol., 68: 4884–4893.

    Article  CAS  PubMed  Google Scholar 

  • Mortimer R., Polsinelli M. (1999). On the origins of wine yeast. Res. Microbiol., 150: 199–204.

    Article  CAS  PubMed  Google Scholar 

  • Ness F., Lavallee F., Dubourdieu D., Aigle M., Dulau L. (1993). Identification of yeast strains using the polymerase chain reaction. J. Sci. Food Agri., 62: 89–94.

    Article  CAS  Google Scholar 

  • Nisiotou A.A., Spiropoulos A.E., Nychas G.J. (2007). Yeast community structures and dynamics in healthy and Botrytisaffected grape must fermentations. Appl. Environ. Microbiol., 73: 6705–6713.

    Article  CAS  PubMed  Google Scholar 

  • Pretorius I.S. (2000). Tailoring wine yeast for the new millennium: novel approaches to the ancient art of winemaking. Yeast, 16: 675–729.

    Article  CAS  PubMed  Google Scholar 

  • Pretorius I.S., Van der Westhuizen T.J., Augustyn O.P.H. (1999). Yeast biodiversity in vineyards and wineries and its importance to the South African wine industry. S. Afr. J. Enol. Vitic., 20: 61–74.

    Google Scholar 

  • Querol A., Jiménez M., Huerta T. (1990). Microbiological and enological parameters during fermentation of musts from poor and normal grape-harvest in the region of Alicante, Spain. J. Food Sci., 55: 1603–1606.

    Article  CAS  Google Scholar 

  • Querol A., Fernandez-Espinar M.T., del Olmo M., Barrio E. (2003). Adaptive evolution of wine yeast. Int. J. Food Microbiol., 86: 3–10.

    Article  CAS  PubMed  Google Scholar 

  • Renouf V., Falcou M., Miot-Sertier C., Perello M.C., DeRevel G., Lonvaud-Funel A. (2006). Interactions betweenBrettanomyces bruxellensis and other yeast species during the initial stages of winemaking. J. Appl. Microbiol., 100: 1208–1219.

    Article  CAS  PubMed  Google Scholar 

  • Schütz M., Gafner J. (1994). Dynamics of the yeast strain population during spontaneous alcoholic fermentation determined by CHEF gel electrophoresis. Lett. Appl. Microbiol., 19: 253–257.

    Article  Google Scholar 

  • Schuller D., Pereira L., Alves H., Cambon B., Dequin S., Casal M. (2007). Genetic characterization of commercialSaccharomyces cerevisiae isolates recovered from vineyard environments. Yeast, 24: 625–636.

    Article  CAS  PubMed  Google Scholar 

  • Sipiczki M., Romano P., Capece A., Paraggio M. (2004). Genetic segregation of naturalSaccharomyces cerevisiae strains derived from spontaneous fermentation of Aglianico wine. J. Appl. Microbiol., 96: 1169–1175.

    Article  CAS  PubMed  Google Scholar 

  • Valero E., Schuller D., Cambon B., Casal M., Dequin S. (2005). Dissemination and survival of commercial wine yeast in the vineyard: a large-scale, three-years study. FEMS Yeast Res., 5: 959–969.

    Article  CAS  PubMed  Google Scholar 

  • Van der Westhuizen T.J., Augustyn O.P.H., Pretorius I.S. (2000a). Geographic distribution of indigenousSaccharomyces cerevisiae strains isolated from vineyards in the coastal regions of the Western Cape in South Africa. S. Afr. J. Enol. Vitic., 21: 3–9.

    Google Scholar 

  • Van der Westhuizen TJ, Augustyn O.P.H., Khan W., Pretorius I.S. (2000b). Seasonal variation of indigenousSaccharomyces cerevisiae strains isolated from Western Cape vineyards in South Africa. S. Afr. J. Enol. Vitic., 21: 10–16.

    Google Scholar 

  • van Keulen H., Lindmark D.G., Zeman K.E., Gerlosky W. (2003). Yeasts present during spontaneous fermentation of Lake Erie Chardonnay, Pinot Gris and Riesling. Antonie van Leeuwenhoek, 83: 149–154.

    Article  PubMed  Google Scholar 

  • Versavaud A., Courcoux P., Roulland C., Dulau L., Hallet J.N. (1995). Genetic diversity and geographical distribution of wildSaccharomyces cerevisiae strains from the wine-producing area of Charentes, France. Appl. Environ. Microbiol., 61: 3521–3529.

    CAS  PubMed  Google Scholar 

  • Wang Q.M., Bai F.Y. (2004). Four new yeast species of the genusSporobolomyces from plant leaves. FEMS Yeast Res., 4: 579–586.

    Article  CAS  PubMed  Google Scholar 

  • White T.J., Bruns T., Lee S., Taylor J. (1990). Amplification and direct sequencing of fungi ribosomal RNA genes for phylogenetics. In: Innis M.A., Gelfand D.H., Sninsky J.J., White T.J., Eds, PCR Protocols: A Guide To Methods And Applications. Academic Press, San Diego, California, pp. 315–322.

    Google Scholar 

  • Yap N.A., de Barros Lopes M., Langridge P., Henschke P.A. (2000). The incidence of killer activity of non-Saccharomyces yeasts towards indigenous yeast species of grape must: potential application in wine fermentation. J. Appl. Microbiol., 89: 381–389.

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. College of Agriculture and Biotechnology, China Agricultural University, 100193, Beijing, China

    Huihui Sun, Huiqin Ma & Meiling Hao

  2. The Australian Wine Research Institute, PO Box 197, 5064, Glen Osmond (Adelaide, SA, Australia

    Isak S. Pretorius

  3. College of Food Science and Nutritional Engineering, China Agricultural University, 100083, Beijing, China

    Shangwu Chen

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  1. Huihui Sun
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  2. Huiqin Ma
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  3. Meiling Hao
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  4. Isak S. Pretorius
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  5. Shangwu Chen
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Correspondence to Shangwu Chen.

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These authors contributed equally to this work.

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Sun, H., Ma, H., Hao, M. et al. Identification of yeast population dynamics of spontaneous fermentation in Beijing wine region, China. Ann. Microbiol. 59, 69–76 (2009). https://doi.org/10.1007/BF03175601

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  • DOI: https://doi.org/10.1007/BF03175601

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