Abstract
Two hundred and six bacterial isolates were obtained from leaf, flower and stem of three healthy plants,Euphorbia pulcherrima Willd,Pyrethrum cinerariifolium Trev. andHeracleum candicans Wall. The nematicidal activity experiment showed that a total of 92 isolates displayed activity againstCaenorhabditis elegans (Maupas) Dougherty, and 70 isolates resulted active againstBursaphelenchus xylophilus (Steiner & Buhrer) Nickle. Strain LCB-3 exhibited the strongest nematicidal activity against both two nematodes. According to the 16S rDNA, the strain LCB-3 was identified asBrevundimonas diminuta. Based on bioassay-guided fractionation, a nematicidal metabolite (R)-(−)-2-ethylhexan-1-ol was obtained from LCB-3. The median lethal concentrations (LC50) of the compound were 542.0 mg l−1 againstC. elegans and 168.1 mg l−1 againstB. xylophilus 48 h after treatment.
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Zheng, L., Li, G., Wang, X. et al. Nematicidal endophytic bacteria obtained from plants. Ann. Microbiol. 58, 569–572 (2008). https://doi.org/10.1007/BF03175559
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DOI: https://doi.org/10.1007/BF03175559