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Cellulose degradation and glucose accumulation byClostridium thermocellum ATCC 27405 under different cultural conditions

Annals of Microbiology volume 57, pages 395–400 (2007)Cite this article

Abstract

Effect of various cultural parameters on cellulose degradation, glucose accumulation and ethanol production byClostridium thermocellum ATCC 27405 were investigated. Optimum pH values for glucose accumulation and ethanol production were determined as 7 and 10, respectively. Highest amount of ethanol (0.92 g/l) was obtained from the culture which contains 10 g urea/l with 34.5% decrease in glucose accumulation. Addition of 100 mM phosphate to the medium increased ethanol production while cellulose degradation and sugar accumulation decreased by 34 and 99%, respectively. Among minerals tested, Mg+2 was found to be the most important element which affects cellulose degradation. When the medium contained no Mg+2, residual cellulose concentration was 4.3 g cellulose/l. When the cultural parameters were optimised, glucose accumulation started at early days of fermentation and glucose concentration was 60% higher than that of the control at the 10th day of fermentation.

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  1. Environmental Engineering Department, Gebze Institute of Technology, 41400, Gebze Kocaeli, Turkey

    Özlem Özpinar & Melek Özkan

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  1. Özlem Özpinar
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  2. Melek Özkan
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Correspondence to Melek Özkan.

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Özpinar, Ö., Özkan, M. Cellulose degradation and glucose accumulation byClostridium thermocellum ATCC 27405 under different cultural conditions. Ann. Microbiol. 57, 395–400 (2007). https://doi.org/10.1007/BF03175079

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  • DOI: https://doi.org/10.1007/BF03175079

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Annals of Microbiology

ISSN: 1869-2044

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