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Effect of recombinant human GH and GHRH on plasma metabolite levels in rainbow trout (Oncorhynchus mykiss)

Efecto de preparados recombinantes de GH y GHRH humanas sobre la concentración plasmática de metabolitos en la trucha

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Abstract

The aim of the present work was to study metabolic changes in rainbow trout treated with preparations of human recombinants of growth hormone (rhGH) and GH-releasing hormone (rhGHRH), by analysing time course variations in plasma metabolite levels. Trouts were given the hormones by intraperitoneal injection in a single dose (rhGH: 0.5 μg/g b.w.; rhGHRH: 0.06μg/g b.w.) or as a weekly injection over a period of 4 weeks. The concentrations of glucose, lactate, free fatty acids and glycerol were determined in plasma immediately before injection and 1, 3, 6, 12, 24, 48 and 168 hours post-injection. Results indicate that glucose and lactate levels increase, while slight variations in free fatty acids was observed after the injection of rhGH and rhGHRH.

Resumen

Se estudian en este trabajo los cambios metabólicos producidos en truchas arco iris tratadas con preparaciones de hormona del crecimiento (rhGH) y de hormona liberadora de la hormona del crecimiento (rhGHRH) recombinantes humanas. Las truchas fueron tratadas por inyección intraperitoneal de las hormonas en una sola dosi (rhGH: 0,5 μg/g peso corporal; rhGHRH: 0,06 μg/g p.c.) o con una inyección semanal durante un periodo de 4 semanas. Se determinaron las concentraciones de glucosa, lactato, ácidos grasos libres y glicerol en el plasma inmediatamente antes de la inyección y a 1, 3, 6, 12, 24, 48 y 168 horas tras la inyección. Los resultados indican un aumento en las concentraciones plasmáticas de glucosa y de lacato y pequeñas variaciones en las de ácidos grasos libres después de la inyección de rhGH y rhGHRH.

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Authors and Affiliations

  1. Department of Physiology, Faculty of Biology, University of Murcia, Campus de Espinardo, 30100, Murcia, Spain

    M. D. Hernández Llorente, M. J. Dato Gómez, J. de Costa Ruiz, P. Mendiola López & S. Zamora Navarro

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  1. M. D. Hernández Llorente
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  2. M. J. Dato Gómez
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  3. J. de Costa Ruiz
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  4. P. Mendiola López
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  5. S. Zamora Navarro
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Correspondence to J. de Costa Ruiz.

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Llorente, M.D.H., Gómez, M.J.D., de Costa Ruiz, J. et al. Effect of recombinant human GH and GHRH on plasma metabolite levels in rainbow trout (Oncorhynchus mykiss). J. Physiol. Biochem. 60, 211–218 (2004). https://doi.org/10.1007/BF03167031

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  • DOI: https://doi.org/10.1007/BF03167031

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