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Rapidin Vitro propagation and enhanced stevioside accumulation inStevia rebaudiana Bert

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Abstract

A very rapid and efficient regeneration method has been established using mature expiants ofStevia rebaudiana Bert. Adventitious shoots were induced from nodal expiants of field-grown plants on four basal media supplemented with various combinations of auxins and cytokinins. The best performance (23.4 ± 2.1 shoots per expiant) was obtained on a Murashige and Skoog (MS) medium containing 2 mg L-1 IAA and 0.5 mg L-1 kinetin. Roots were then produced when thesein vitro- regenerated shoots were transferred to an MS medium supplemented with 3% sucrose and 2 mg L-1 IBA. When acclimatized to soil, the rooted plants had a 98.4% survival rate. Following transplantation in the field, stevioside contents were similar between the regenerated plants (10.68 mg g-1 dry weight) and the mother plants (12.01 mg g-1 dw).

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  1. Department of Oriental Medicine Materials, Dongshin University, 520-714, Naju, Korea

    Sung Jin Hwang

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  1. Sung Jin Hwang
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Correspondence to Sung Jin Hwang.

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Hwang, S.J. Rapidin Vitro propagation and enhanced stevioside accumulation inStevia rebaudiana Bert. J. Plant Biol. 49, 267–270 (2006). https://doi.org/10.1007/BF03031153

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  • DOI: https://doi.org/10.1007/BF03031153

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