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Long-term effects of several biochemical and physical factors on cellulose biosynthesis in callus and suspension cultures of normal and mutant barley (Hordeum vulgare L.) strains producing less cellulose

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Abstract

To clarify a low level of cellulose biosynthesis of thein vitro cultured cells, the effects of several biochemical factors such as carbon sources (sucrose, maltose, and UDPG), antioxidants (ascorbic acid and glutathione) and physical factors such as artificial pressure, high gravity, on the cellulose production in barley callus and suspension cultures were investigated. In the suspension culture of two barley strains, the supplement of different concentrations (0, 1.5, 3.0, and 4.5%) of sucrose or maltose into the medium for 30 days did not promote the cellulose production and 4.5% of sugar supplement was rather inhibitory in one strain. However, in the presence of sucrose at 3%, UDPG (3 or 10 mM) supplement, as a precursor for cellulose, promoted 1.2–13 fold of the production in two strains. A low concentration (3 mM) of ascorbic acid and glutathione promoted 1.5 and 1.2 fold of the production in two strains, respectively. These results suggest that low cellulose biosynthesis of thein vitro cultured cells is due to a decreased level of the UDPG in the cytosol, and that the oxidative condition of external medium impedes cellulose synthesis in some manners. Artificial pressure applied to the callus promoted 1.4 fold of the cellulose production. High gravity (5,000 or 10,000g) applied to the suspension-cultured cells by centrifugation did not cause a substantial change.

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Authors and Affiliations

  1. Faculty of Biological Sciences, College of Natural Sciences, Chonbuk National University, 561-756, Chonju, Korea

    Up-Dong Yeo & Woong-Young Soh

  2. Faculty of Integrated Arts and Sciences, Hiroshima University, 739, Higashi Hiroshima, Japan

    Naoki Nakagawa & Naoki Sakurai

  3. Research Institute for Bioresources, Okayama University, 710, Kurashiki, Okayama, Japan

    Kazuyoshi Takeda

Authors
  1. Up-Dong Yeo
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  2. Woong-Young Soh
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  3. Naoki Nakagawa
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  4. Naoki Sakurai
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  5. Kazuyoshi Takeda
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Yeo, UD., Soh, WY., Nakagawa, N. et al. Long-term effects of several biochemical and physical factors on cellulose biosynthesis in callus and suspension cultures of normal and mutant barley (Hordeum vulgare L.) strains producing less cellulose. J. Plant Biol. 41, 219–226 (1998). https://doi.org/10.1007/BF03030256

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  • DOI: https://doi.org/10.1007/BF03030256

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