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Analysis of carbon metabolism in transgenicArabidopsis thaliana transformed with the cyanobacterial sucrose phosphate synthase gene

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Abstract

To investigate the effects of sucrose-phosphate synthase (SPS) on carbon partitioning, transgenicArabidopsis plants transformed withSynechocystis SPS were constructed. The integration, copy number and expression level were confirmed by Southern and Northern blot analyses. SPS activity in leaves from the transgenic and wild type plants was not significantly different. The level of sucrose and starch in the leaves of transgenic plant were slightly decreased compared to wild type. The glucose and fructose contents were increased up to two-fold compared to wild type during the light period. It is our speculation that the decreased sucrose level of the transgenic plant might be caused by the high acid invertase.

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Authors and Affiliations

  1. Plant Metabolism Research Center & Graduate School of Biotechnology, Kyung Hee University, 449-701, Suwon, Korea

    Seok-Yoon Yoon, Jang-Wook Lee, Seong-Hee Bhoo, Jong-Seong Jeon, Youn-Hyung Lee & Tae Ryong Hahn

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  1. Seok-Yoon Yoon
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  2. Jang-Wook Lee
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  3. Seong-Hee Bhoo
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  4. Jong-Seong Jeon
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  5. Youn-Hyung Lee
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  6. Tae Ryong Hahn
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Correspondence to Tae Ryong Hahn.

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These authors contributed equally to this work

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Yoon, SY., Lee, JW., Bhoo, SH. et al. Analysis of carbon metabolism in transgenicArabidopsis thaliana transformed with the cyanobacterial sucrose phosphate synthase gene. J. Plant Biol. 47, 42–47 (2004). https://doi.org/10.1007/BF03030226

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  • DOI: https://doi.org/10.1007/BF03030226

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