Abstract
We earlier reported that CM-AIa isolated fromChelidonium majus had mitogenic activity, generated lymphokine-activated killer cells, and increased the number of granulocyte-macrophage colony-forming cells (GM-CFC). In an extended effort to search for other immunostimulatory effects, we evaluated the protective effects of in vivo injected CM-AIa against irradiation. CM-AIa was found to increase the number of bone marrow cells, spleen cells, GM-CFC, and platelets in irradiated mice. In addition, this agent induced endogenous production of cytokines such as interleukin 1 and tumor necrosis factor a, which are required for hematopoietic recovery. We also demonstrated that CM-AIa treatment 24 hours before irradiation protected mice with 80% survival at lethal dose 100/15. These findings indicate that CM-AIa may be a useful agent for reducing the time needed for reconstitution of hematopoietic cells after irradiation treatment.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Chirigos MA, Mastrangelo ML, Mitchell M, Krim M.Progress in Cancer Research and Therapy: Mediation of Cellular Immunity in Cancer by Immune Modifier. Vol 19. New York: Raven; 1982.
Hersh EM, Chirigos MA, Masrtangelo M J.Progress in Cancer Research and Therapy: Augmenting Agents in Cancer Therapy. Vol 16. New York: Raven; 1981.
Blackett NM, Marsh JC, Gordon MY, Okell SF, Aguado M. Simultaneous assay by six methods of the effect of haemopoietic precursor cells of adriamycin, methyl-CCNU,60Co γ-rays, vinblastine, and cytosine arabinoside.Exp Hematol. 1978;6:2–8.
Elkind MM. Repair processes in radiation biology.Radiat Res. 1984;100:425–429.
Behling UH. The radioprotective effect of bacterial endotoxin. In: NowotnyA, ed.Beneficial Effects of Endotoxin. New York: Plenum; 1983.
Song JY, Yang HO, Pyo SN, Jung IS, Yi SY, Yun YS. Immunomodulatory activity of protein-bound polysaccharide extracted fromChelidonium majus.Arch Pharm Res. 2002;25:158–164.
Till JE, McCulloch EA. Early repair processes in marrow cells irradiated and proliferating in vivo.Radiat Res. 1963;18:96–99.
Talmadge JE, Herberman RB. The preclinical screening laboratory: evaluation of immunomodulatory and therapeutic properties of biological response modifiers.Cancer Treat Rep. 1986:70:171–182.
Prasard KN.Handbook of Radiology. Boca Raton FL: CRC Press; 1984.
Ross WM, Peeke J. Radioprotection conferred by dextran sulfate given before irradiation in mice.Exp Hematol. 1986;14:147–155.
Ainsworth EJ. From endotoxins to newer immunomodulators: survival-promoting effects of microbial polysaccharide complexes in irradiated animals.Pharmacol Ther. 1988;39:223–241.
Patchen ML, MacVittie TJ. Stimulated hemopoiesis and enhanced survival following glucan treatment in sublethally and lethally irradiated mice.Int J Immunopharmacol. 1985;7:923–932.
Uglyanitsa KN, Nefydov LI, Doroshenko YM, et al. Ukrain: a novel antitumor drug.Drugs Exp Clin Res. 2000;16:341–456.
Santavy F, Alkaloids F. In: StahlE, ed.Thin-Layer Chromatography. Berlin: Springer; 1973:421–471.
Kalechman Y, Zuloff A, Albeck M, Strassmann G, Sredni B. Role of endogenous cytokines secretion in radioprotection conferred by the immunomodulator ammonium trichloro(dioxyethylene-O- O′)tellurate.Blood. 1995;85:1555–1561.
Nakamura W, Kankura T, Eto H. Occult blood appearance in feces and tissue hemorrhage in mice after whole body x-irradiation.Radiat Res. 1971;48:169–178.
Nakamura W. Effect of thrombocytes-rich plasma on x-ray induced bone marrow death in mice.Radiat Res. 1973;55:118–128.
Neta R, Douches SD, Oppenheim JJ. Interleukin 1 is a radioprotector.J Immunol. 1986;136:2483–2485.
Neta R, Oppenheim JJ, Douches SD. Interdependence of the radioprotective effects of human recombinant interleukin-1 alpha, tumor necrosis factor alpha, granulocyte-colony stimulating factor, and murine recombinant granulocyte-colony stimulating factor.J Immunol. 1988;140:108–111.
Neta R, Vogel SN, Sipe JD, Wong GG, Nordan RP Comparison of in vivo effects of human recombinant IL-1 and human recombinant IL-6 in mice.Lymphokine Res. 1988;7:403–412.
Metcalf D, Nicola NA. Direct proliferative actions of stem cell factor on murine bone marrow cells in vitro: effects of combination with colony stimulating factors.Proc NatlAcad Sci USA. 1991;88:6239–62433.
Neta R, Perlstein R, Vogel SN, Ledney GD, Abrams J. Role of interleukin 6 (IL-6) in protection from lethal irradiation and in endocrine responses to IL-1 and tumor necrosis factor.J Exp Med. 1992;175:689–694.
Vogel SN, Douches SD, Kaufman EN, Neta R. Induction of colony stimulating factor in vivo by recombinant interleukin-1 alpha and recombinant tumor necrosis factor alpha.J Immunol. 1987;138:2143–21488.
Kinnamon KE, Ketterling LL, Stampfli HF, Grenan MM. Mouse endogenous spleen counts as a means of screening for anti-radiation drug.Proc Soc Exp Biol Med. 1980;164:370–373.
Neta R, Sztein MB, Oppenheim JJ, Gillis S, Douches SD. The in vivo effects of interleukin-1,I: bone marrow cells induced to cycle after administration of interleukin-1.J Immunol. 1987;139:1861–1866.
Metcalf D. The molecular biology and functions of the granulocytes macrophage colony stimulating factors.Blood. 1986;67:257–267.
Metcalf D, Moore MAS.Haemopoietic Cells. Amsterdam: North Holland; 1971.Frontiers in Biology; vol 24.
Author information
Authors and Affiliations
Corresponding author
About this article
Cite this article
Song, JY., Yang, HO., Shim, JY. et al. Radiation Protective Effect of an Extract fromChelidonium majus . Int J Hematol 78, 226–232 (2003). https://doi.org/10.1007/BF02983799
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF02983799