Abstract
Evaluation of the activity of the cold expeller neem oil(Azadirachta indica A. Juss.) and the fractions derived through solvent partitioning, againstDrechslera oryzae, Fusarium oxysporum andAlternaria tenuis showed that the active antifungal fraction is a mixture of tetranortriterpenoids. Further, testing the triterpenoidal mixture derived from the 90% methanol (MeOH) extract of neem oil against 13 phytopathogenic fungi revealed that various species were inhibited to different degrees. Direct preparative High Performance Liquid Chromatography (HPLC) of the active fractions and subsequent bioassay of the semi-pure fractions indicated that the active fractions contained major compounds such as 6-deacetylnimbin, azadiradione, nimbin, salannin and epoxyazadiradione. Pure azadiradione, nimbin, salannin and epoxy-azadiradione did not have appreciable activity. However, when these terpenoids were mixed and bioassayed, they showed antifungal activity, indicating possible additive/synergistic effects.
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References
Devkumar, C. and Sukh Dev. (1993) Chemistry,in: Randhawa, N.S. and Parmar, B.S. [Eds.] Neem Research and Development. Society of Pesticide Science, New Delhi, India, pp. 63–96.
Govindachari, T.R., Gopalakrishnan, G. and Suresh, G. (1996) Isolation of various azadirachtins from neem oil by preparative high performance liquid chromatography.J. Liq. Chromtogr. & Rel. Technol. 19:1729–1733.
Govindachari, T.R., Suresh, G. and Gopalakrishnan, G. (1995) A direct preparative high performance liquid chromatography procedure for the isolation of major triterpenoids and their quantitative determination in neem oil.J. Liq. Chromatogr. 18:3465–3471.
Grayer, R.J. and Harborne, J.B. (1994) A survey of antifungal compounds from higher plants, 1982–1993.Phytochemistry 37:19–42.
Khan, M.W., Alam, M.M., Khan, A.M. and Saxena, S.K. (1974) Effect of water soluble fractions of oil cakes and bitter principles of neem on some fungi and nematodes.Acta Bot. Indica 2:120- 128.
Locke, J.C. (1995) Fungi,in: Schmutterer, H. [Ed.] The Neem Tree, Source of Unique Natural Products for Integrated Pest Management, Medicine, Industry and Other Purposes. VCH, Weinheim, Germany, pp. 118–125.
Parveen, G. and Alam, M.M. (1993) Bioactivity against plant pathogens,in: Randhawa, N.S. and Parmar, B.S. [Eds.] Neem Research and Development. Society of Pesticide Science, New Delhi, India, pp. 144–153.
Paulus, W. (1993) Microbicides for the Protection of Materials. A Handbook. Chapman and Hall, London, UK.
Rukmini, C. (1987) Chemical and nutritional evaluation of neem oil.Food Chem. 42:119–124.
Steinhauer, B. (1994) Antifungal compounds fromAzadirachta indica.in: Kleeberg, H. [Ed.] Practice Oriented Results on Use and Production of Neem Ingredients and Pheromones. 3rd Workshop (Wetzlar, Germany), pp. 93–97. Druck & Graphic, Giessen, Germany.
Steinhauer, B. (1996) Fungicidal activity of commercial neem products,in: Kleeberg, H. and Micheletti, V. [Eds.] Practice Oriented Results on Use and Production of Neem Ingredients and Pheromones. IVth Workshop (Bordighera, Italy), pp. 105–116. Druck & Graphic, Giessen, Germany.
Suresh, G., Narasimhan, N.S., Masilamani, S., Partho, P.D. and Gopalakrishnan, G. (1997) Antifungal fractions and compounds from uncrushed green leaves ofAzadirachta indica.Phytoparasitica 25:33–39.
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Govindachari, T.R., Suresh, G., Gopalakrishnan, G. et al. Identification of antifungal compounds from the seed oil ofAzadirachta Indica . Phytoparasitica 26, 109–116 (1998). https://doi.org/10.1007/BF02980677
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DOI: https://doi.org/10.1007/BF02980677