Abstract
A new monoterpene glycoside, 6’-O-β-D-glucopyranosylalbiflorin (1), and four known compounds; albiflorin (2), 6’-O-benzoylalbiflorin (3), paeoniflorin (4) and benzoyl paeoniflorin (5), were isolated from the methanolic extract of the roots ofPaeonia lactiflora Pall.. Their chemical structures were completely elucidated using a combination of 2D NMR techniques (COSY, HMQC and HMBC) and HRESI-MS analyses. To investigate the bioactivities of these compounds, their effects on the differentiation of osteoblastic MC3T3-E1 cells were tested. Compound 1 (0.01-10 μM) significantly increased the alkaline phosphatase activity and nodules mineralization of MC3T3-E1 cells compared to those of the control (P<0.05). These results suggest that newly isolated compound 1 has a direct stimulatory effect on bone formationin vitro and may contribute to the prevention for osteoporosis.
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Yen, P.H., Van Kiem, P., Nhiem, N.X. et al. A new monoterpene glycoside from the roots ofPaeonia lacti- flora increases the differentiation of osteoblastic MC3T3-E1 cells. Arch. Pharm. Res. 30, 1179–1185 (2007). https://doi.org/10.1007/BF02980258
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DOI: https://doi.org/10.1007/BF02980258