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Triterpenoids and diarylheptanoids from alnus hirsuta inhibit HIF-1 in ags cells

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Abstract

Bioassay-guided fractionation and purification of the EtOAc soluble fraction from the MeOH extract of the stem bark of Alnus hirsuta (Betulaceae), using an in vitro HIF-1 assay, led to the isolation of four triterpenoids (1–4) and six diarylheptanoids (5–10). Their structures were determined by comparison with the physicochemical and spectroscopic data in the literature. These compounds were investigated for their effects on the hypoxia-induced HIF-1 activation using an HIF-1 a mediated reporter gene assay in AGS cells. Among them, two diarylheptanoids, 2-oxatrycyclo[13.2.2.13,7]eicosa-3,5,7(20),15,17,18-hexaen-10-16-diol (6) and 2-oxatrycyclo [13.2.2.13,7]eicosa-3,5,7-(20), 15, 17, 18-hexaen-10-one (7), inhibited HIF-1 activation dose-dependently with IC50 values of 11.2 μM and 12.3 μM, respectively. These two compounds had no significant cytotoxicity to the AGS cells at the effective concentration for the inhibition of HIF-1 activation.

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Authors and Affiliations

  1. College of Pharmacy, Chungnam National University, 305-764, Daejeon, Korea

    WenYi Jin, Xing Fu Cai, MinKyun Na, Jung Joon Lee & KiHwan Bae

  2. Korea Research Institute of Bioscience and Biotechnology, 305-600, Daejeon, Korea

    Xing Fu Cai, MinKyun Na & Jung Joon Lee

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  1. WenYi Jin
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  2. Xing Fu Cai
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  3. MinKyun Na
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  4. Jung Joon Lee
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Correspondence to KiHwan Bae.

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Jin, W., Cai, X.F., Na, M. et al. Triterpenoids and diarylheptanoids from alnus hirsuta inhibit HIF-1 in ags cells. Arch Pharm Res 30, 412–418 (2007). https://doi.org/10.1007/BF02980213

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  • DOI: https://doi.org/10.1007/BF02980213

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