Abstract
Achromobacter xylosoxidans KF701 andPseudomonas putida (NAH7) were significantly different in degradative capability of aromatic compounds including benzoates. biphenyls, and naphthalene. However, both of the bacterial strains can grown on catechol as the sole carbon and energy source. Catechol 2,3-dioxygenase gene for naphthalene oxidation or biphenyl oxidation was cloned intoEscherichia coli HB101 from NAH7 megaplasmid ofP. putida or chromosomal DNA ofA. xylosoxidans KF701. AE. coli HB101 clone containing catechol 2,3-dioxygenase gene fromP. putida (NAH7) contains a recombinant plasmid with 3,6-kb pBR322 and 6-kb insert DNA. AnotherE. coli HB101 clone containing catechol 2,3-dioxygenase gene fromA. xylosoxidans KF701 has a recombinant plasmid with 4.4-kb pBR322 and 10-kb insert DNA. Physical maps of the recombinant plasmids were constructed, and catechol 2,3-dioxygenase gene in the recombinant plasmid was further localized and subcloned into M13. The cloned-catechol 2,3-dioxygenase gene products were identified as yellow bands on nondenaturaing polyacrylamide gel after electrophoresis followed by activity staining with catechol solution.
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Literature Cited
Chaudhry, G. R. and Chapalamadugu, S.: Biodegradation of halogenated organic compounds.Microbiological Rev 55, 59 (1991).
Reiner, A. M.: Metabolism of aromatic compounds in bacteria.J. Biol. Chem. 247, 4960 (1972).
Sander, P., Wittich, R.-M., Fortnagel, P., Wilkes, H. and Francke, W.: Degradation of 1,2,4-trichloro- and 1,2,4,5-tetrachlorobenzene byPseudomonas strains.App. Env. Microbiol.,57, 1430 (1991).
Stanier, R. Y. and Ornston, L. N.: The β-ketoadipate pathway. InAdvances and Microbial Physiology, ed., Rose, A.H. and Tempest, D.W.: Academic press 89 (1973).
Sala-Trepat, J. M. and Evans, W. C.: Themeta cleavage of catechol byAzotobacter species.Eur. J. Biochem. 20, 400 (1971).
Kim, Y., Choi, B., Min, K. R. and Kim, C.-K.: Cloning of catechol 2,3-dioxygenase gene fromPseudomonas putida.Kor. J. Microbiol. 29, 155 (1991).
Birnboim, H. C. and Doly, J.: A rapid alkaline extraction procedure for scrreening plasmid DNA.Nucleic Acids Res.7, 1513 (1979).
Jonston, J. B. and Gunsalus, I. C.: Isolation of metabolic plasmid DNA fromPseudomonas putida.Biochem. Biophys. Res. Comm. 75, 13 (1977).
Maniatis, T., Fritsch, E. F. and Sambrook, J.: Molecular cloning: a laboratory manual, Cold Spring Harbor Laboratory (1982).
Weber, K. and Osborn, M.: Proteins and sodium dodecyl sulfate: molecular weight determination on polyacrylamide gels and related procedures. InProteins, ed., Neurath, H. and Robert, L.: Academic press3, 179 (1975).
Furukawa, K., Hayase, N., Taira, K. and Tomizuka, N.: Molecular relationship of chromosomal genes encoding biphenyls/polychlorinated biphenyl catabolism: some soil bacteria possess highly conservedbph operon.J. Bacteriol. 171, 5467 (1989).
Schell, M. A.: Cloning and expression inE. coli of the naphthalene degradation gens from plasmid NAH7.J. Bacteriol. 153, 822 (1983).
Yen, K.-M., and Gunsalus, I. C.: Plasmid gene organization: naphthalene/salicylate oxidation.Proc. Natl. Acad. Sci. USA 79, 874 (1982).
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Kim, Y., Choi, B. & Min, K.R. Molecular cloning and M13 subcloning of genes encoding catechol dioxygenases. Arch. Pharm. Res. 15, 48–51 (1992). https://doi.org/10.1007/BF02973983
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DOI: https://doi.org/10.1007/BF02973983