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Evidence for lipopolysaccharideinduced differentiation of RAW264.7 murine macrophage cell line into dendritic like cells

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Abstract

Effect of lipopolysaccharide (LPS) on RAW264.7 macrophage cell line was studied. LPS-treated RAW264.7 cells increased in cell size and acquired distinct dendritic morphology. At the optimal dose of LPS (1 μg/ml), almost 70% RAW264.7 cells acquired dendritic morphology. Flow cytometric studies indicate that the cell surface markers known to be expressed on dendritic cells and involved in antigen presentation and T cell activation (B7.1, B7.2, CD40, MHC class II antigens and CD1d) were also markedly upregulated on LPS-treated RAW264.7 cells. Our results suggest the possibility that LPS by itself could constitute a sufficient signal for differentiation of macrophages into DC-like cells.

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Abbreviations

DCs:

Dendritic cells

LBP:

lipopolysaccharide binding protein

LPS:

lipopolysaccharide

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Authors and Affiliations

  1. School of Life Sciences, Jawaharlal Nehru University, 110 067, New Delhi, India

    Rajiv K. Saxena

  2. Analytical Services Branch, HELD, National Institute for Occupational Safety and Health, Center of Disease Control and Prevention, 26506, Morgantown, WV, USA

    Rajiv K. Saxena, Val Vallyathan & Daniel M. Lewis

Authors
  1. Rajiv K. Saxena
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  2. Val Vallyathan
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  3. Daniel M. Lewis
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Correspondence to Rajiv K. Saxena.

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Saxena, R.K., Vallyathan, V. & Lewis, D.M. Evidence for lipopolysaccharideinduced differentiation of RAW264.7 murine macrophage cell line into dendritic like cells. J. Biosci. 28, 129–134 (2003). https://doi.org/10.1007/BF02970143

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