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Modulation of polymorphonuclear leukocytes function by incubation with human serum from oxidant-challenged individuals

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Abstract

Polymorphonuclear leukocytes (PMN) from healthy donors were tested for stimulated release of superoxide anions after being incubated with serum of welders and of a group of unexposed individuals. These two groups were further subdivided either according to age or to smoking habits. The experiments showed that stimulated superoxide production from PMN was inhibited(P 005) by serum from young smokers as compared to that of young nonsmokers, both from the unexposed group. Incubation of PMN with serum from elderly nonsmoking individuals decreased superoxide production as compared to incubation with serum from young nonsmoking individuals, both from the unexposed group. A decrease in superoxide production by incubation with serum of welders as compared to that of unexposed individuals was significant only when the comparison was carried out between the young, non-smoking subgroups. These findings suggest that age, smoking, and exposure to oxidants induce appearance in serum of factors that affect the PMN function.

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Abbreviations

CABG:

Coronary artery bypass grafting

FEV:

forced expiratory volume

FVC:

forced vital capacity

PMN:

polymorphonuclear leukocytes

References

  • Aloufy A, Lerman Y, Tamir A and Hoffer E 2001 Superoxide anion release by peripheral polymorphonuclear leukocytes in welders;Int. Arch. Occup. Environ. Health 74 450–453

    Article  CAS  Google Scholar 

  • Bugajski P, Kalawski R, Balinski M, Wysocki H, Olszewski R, Szczepanik A and Siminiak T 1999 Plasma-mediated stimulation of neutrophil superoxide anion production during coronary artery bypass grafting: role of endothelin-1;Thorac. Cardiovasc. Surg. 47 144–147

    Article  CAS  Google Scholar 

  • Di Lorenzo G, Balistreri C R, Candore G, Cigna D, Colombo A, Romano G C, Colucci A T, Gervasi F, Potestio M and Caruso C 1999 Granulocyte and natural killer activity in the elderly;Mech. Age. Dev. 108 25–38

    Article  Google Scholar 

  • Governa M, Valentino M, Visona I and Scielso R 1988 Impairment of chemotaxis of polymorphonuclear leukocytes from lead acid battery workers;Sci. Total Environ. 71 543–546

    Article  CAS  Google Scholar 

  • Governa M, Valentino M, Visona I and Monaco F 1994 Human polymorphonuclear leukocyte chemotaxis as a tool on detecting biological early effects in workers occupationally exposed to low levels of n-Hexane;Hum. Exp. Toxicol. 13 663–670

    Article  CAS  Google Scholar 

  • Perlingeiro R C and Queiroz M L 1994 Polymorphonuclear phagocytosis and killing in workers exposed to inorganic mercury;Int. J. Immunopharmacol. 16 1011–1017

    Article  CAS  Google Scholar 

  • Piazzolla G, Tortorella C, Serrone M, Jirillo E and Antonaci S 1998Modulation of cytoskeleton assembly capacity and oxidative response in aged neutrophils;Immunopharmacol. Immunotoxicol. 20 251–266

    Article  CAS  Google Scholar 

  • Pick E and Mizel D 1981 Rapid microassays for the measurement of superoxide and hydrogen peroxide production by macrophages in culture using an autoenzyme immunoassay reader;J. Immunol. Methods 46 211–226

    Article  CAS  Google Scholar 

  • Polignano A, Tortorella C, Venezia A, Jirillo E and Antonaci S 1994 Age-associated changes of neutrophil responsiveness in a human healthy elderly population;Cytobios 80 145–153

    CAS  PubMed  Google Scholar 

  • Szücs S, Varga C, Ember I and Kertai P 1994 The separation of the granulocytes from different rat strains;J. Immunol. Methods 167 245–251

    Article  Google Scholar 

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Authors and Affiliations

  1. Israel Poison Information Center, Rambam Medical Center, 31096, Haifa, Israel

    E. Hoffer, A. Tabak & Y. Baum

  2. Kupat Holim Clalit, Occupational Medicine, Faculty of Medicine, 31096, Technion, Haifa, Israel

    T. Machamid

  3. Department of Epidemiology, Faculty of Medicine, 31096, Technion, Haifa, Israel

    A. Tamir

  4. Occupational Health Center, Kupat Holim Clalit and the Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv, Israel

    Y. Lerman

Authors
  1. E. Hoffer
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  2. T. Machamid
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  3. A. Tabak
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  4. Y. Baum
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  5. A. Tamir
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  6. Y. Lerman
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Correspondence to E. Hoffer.

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Hoffer, E., Machamid, T., Tabak, A. et al. Modulation of polymorphonuclear leukocytes function by incubation with human serum from oxidant-challenged individuals. J. Biosci. 28, 91–94 (2003). https://doi.org/10.1007/BF02970137

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  • DOI: https://doi.org/10.1007/BF02970137

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