Skip to main content
SpringerLink
Log in

Bestimmung von DON-3-Glukosid in künstlich und natürlich kontaminiertem Weizen mittels LC-MS/MS

Determination of DON-3-Glucoside in artificially and naturally contaminated wheat with LC-MS/MS

  • Published:
Mycotoxin Research Aims and scope Submit manuscript

Abstract

Naturally contaminated and artificiallyFusarium spp. inoculated wheat was analyzed for deoxynivalenol, deoxynivalenol-3-glucoside, 3-acetyl-deoxynivalenol and 15-acetyl-deoxynivalenol. After extraction and clean-up with MycoSep columns, the trichothecenes were determined using a LC-ESI-MS/MS method. Deoxynivalenol-3-glucoside was detectable in 4 out of 4 artificially inoculated and in 22 out of 25 naturally contaminated wheat samples. For the latter, the average relative deoxynivalenol-3-glucoside concentration was about 6% of the deoxynivalenol concentration. The maximum relative deoxynivalenol-3-glucoside concentration was 12% as compared to the concentration of deoxynivalenol. In all samples, the concentration of deoxynivalenol-3-glucoside was higher than the concentrations of 3-acetyl-deoxynivalenol or 15-acetyl-deoxynivalenol.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literatur

  1. Berthiller F, Schuhmacher R, Buttinger G, Freudenschuss M, Adam G, Krska R (2003) Synthesis of Deoxynivalenol-Glucosides and their Characterization using a QTrap LC-MS/MS. Mycotoxin Research 19 (1): 47–50

    Article  CAS  Google Scholar 

  2. Dall'Asta C, Berthiller F, Schuhmacher R, Adam G, Lemmens M, Krska R (2005) DON-Glycosides: Characterisation of Synthesis Products and Screening for their Occurrence in DON-treated Wheat Samples. Mycotoxin Research 21 (2): 123–127

    Article  Google Scholar 

  3. Berthiller F, Dall'Asta C, Schuhmacher R, Lemmens M, Adam G, Krska R (2005) Masked Mycotoxins: Determination of a Deoxynivalenol Glucoside in Artificially and Naturally Contaminated Wheat by LC-MS/MS. J Agric Food Chem 53: 3421–3425

    Article  PubMed  CAS  Google Scholar 

  4. Bürstmayr H, Hartl L, Griesser M, Angerer N, Lengauer D, Miedaner T, Steiner B, Lemmens M (2003) Molecular Mapping of QTL forFusarium Head Blight Resistance in Wheat II. Combined Type 1 and Type II Resistance. Theor Appl Genet 107: 503–508

    Article  Google Scholar 

  5. Berthiller F, Schuhmacher R, Buttinger G, Krska R (2005) Rapid Simultaneous Determination of Major Type A-and B-Trichothecenes as well as Zearalenone in Maize by High-Performance Liquid Chromatography — Tandem Mass Spectrometry. J Chrom A 1062: 209–216

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Christian Doppler Laboratory for Mycotoxin Research, Department for Agrobiotechnology (IFA-Tulln), University of Natural Resources and Applied Life Sciences, Vienna, Konrad Lorenz Strasse 20, 3430, Tulln, Austria

    F. Berthiller, R. Krska & R. Schuhmacher

  2. Department of Organic and Industrial Chemistry, University of Parma, Parco Area Scienze 17/A, Parma, Italy

    C. Dall'Asta

  3. Institute for Biotechnology in Plant Production Department for Agrobiotechnology (IFA-Tulln), University of Natural Resources and Applied Life Sciences, Vienna, Konrad Lorenz Strasse 20, 3430, Tulln, Austria

    M. Lemmens

  4. Institute of Applied Genetics and Cell Biology, Department of Applied Plant Sciences and Plant Biotechnology, University of Natural Resources and Applied Life Sciences, Vienna, Muthgasse 18, 1190, Vienna, Austria

    G. Adam

Authors
  1. F. Berthiller
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. Krska
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. C. Dall'Asta
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. Lemmens
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. G. Adam
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. R. Schuhmacher
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to R. Krska.

Additional information

Financial support: Christian Doppler Society, Austrian Genome Research Intiative (GEN-AU)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Berthiller, F., Krska, R., Dall'Asta, C. et al. Bestimmung von DON-3-Glukosid in künstlich und natürlich kontaminiertem Weizen mittels LC-MS/MS. Mycotox Res 21, 205–208 (2005). https://doi.org/10.1007/BF02959264

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02959264

Keywords

Search

Navigation