Skip to main content
SpringerLink
Log in

Development of deoxynivalenol contents in relation to the PCR detection of potentially trichothecene producingFusarium spp. during storage of wheat

  • Published:
Mycotoxin Research Aims and scope Submit manuscript

Abstract

Development of deoxynivalenol (DON) in wheat with a low contamination withFusarium spp. was investigated under suboptimal storage conditions (17% and 20% grain moisture, 20°C). The influence of storage on the relative DNA content of potential DON producers was also determined. The DON contents were quantified using an ELISA. The Tox5 PCR was used for the detection of potential trichothecene producers and for the estimation of their relative DNA content. ThegaoA gene was subsequently amplified by PCR to detect specificallyFusarium graminearum. The concentration ofF. graminearum DNA was semiquantitatively determined using a Light Cycler™. The DON concentrations increased during storage trials but the intensity of PCR signals decreased.

Zusammenfassung

Die Bildung von Deoxynivalenol (DON) in Weizen wurde, ausgehend von einem geringen Besatz mitFusarium spp., unter suboptimalen Lagerbedingungen (17% und 20% Kornfeuchte, 20°C) untersucht. Der Einfluss der Lagerung auf die relative DNA-Masse potentieller DON-Bildner wurde ebenso ermittelt. Die DON-Gehalte wurden mittels ELISA quantifiziert. Die Tox5-PCR wurde zur Detektion potentieller Trichothecen-Bildner und zur Abschätzung ihrer relativen DNA-Masse eingesetzt. DasgaoA-Gen wurde anschließend mit der PCR amplifiziert, um spezifischFusarium graminearum nachzuweisen. Die Konzentration derF. graminearum-DNA wurde im Light Cycler™ semiquantitativ bestimmt. Die DON-Konzentrationen nahmen im Verlauf der Lagerungsversuche zu, während die Intensität der PCR-Signale abnahm.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Birzele B, Prange A, Krämer J (2000) Deoxynivalenol and ochratoxin A in German wheat and changes of level in relation to storage parameters. Food Add. Contam. (in press)

  2. Doohan FM, Weston G, Rezanoor HM, Parry DW, Nicholson P (1999) Development and use of a reverse transcription-PCR assay to study expression oftri5 byFusarium speciesin vitro andin planta. Appl. Environ. Microbiol. 65: 3850–3854

    PubMed  CAS  Google Scholar 

  3. Müller H-M, Reimann J, Schumacher U, Schwadorf K (1998) Natural occurrence ofFusarium toxins in oats harvested during five years in an area of southwest Germany. Food Add. Contam. 15: 801–806

    Google Scholar 

  4. Niessen ML, Vogel RF (1997) Specific identification ofFusarium graminearum by PCR withgaoA targeted primers. System. Appl. Microbiol. 20: 111–113

    CAS  Google Scholar 

  5. Niessen ML, Vogel RF (1998) Group specific PCR-detection of potential trichothecene-producingFusarium-species in pure cultures and cereal samples. System. Appl. Microbiol. 21: 618–631

    CAS  Google Scholar 

  6. Rotter BA, Prelusky DB, Pestka JJ (1996) Toxicology of deoxynivalenol (vomitoxin). J. Toxicol. Environ. Health 48: 1–34

    Article  PubMed  CAS  Google Scholar 

  7. Sambrook J, Fritsch EF, Maniatis T (Eds) (1989) Molecular cloning: a laboratory manual. Cold Spring Harbour Laboratory, New York

    Google Scholar 

  8. Strehler A (1993) Getreidetrocknung und -lagerung. In: Hydro Agri Dülmen GmbH (Ed) Faustzahlen für Landwirtschaft und Gartenbau, Landwirtschaftsverlag, Münster-Hiltrup, pp. 512–519

Download references

Author information

Authors and Affiliations

  1. Department of Agricultural and Food Microbiology, Institute for Plant Diseases, University of Bonn, Meckenheimer Allee 168, 53115, Bonn, Germany

    Barbara Birzele, Alexander Prange & Johannes Krämer

  2. Institute for Microbiology & Biotechnology, University of Bonn, Meckenheimer Allee 168, 53115, Bonn, Germany

    Alexander Prange

  3. GEN-IAL GmbH, Mülheimer Straße, Tor 3, 53840, Troisdorf, Germany

    Jutta Schönling

Authors
  1. Barbara Birzele
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Alexander Prange
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jutta Schönling
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Johannes Krämer
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Birzele, B., Prange, A., Schönling, J. et al. Development of deoxynivalenol contents in relation to the PCR detection of potentially trichothecene producingFusarium spp. during storage of wheat. Mycotox Res 16 (Suppl 1), 46–49 (2000). https://doi.org/10.1007/BF02942979

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02942979

Keywords

Search

Navigation