Abstract
Anin vitro degradation of bilirubin by isolated human granulocytes and lymphocytes is described. Changes in the bilirubin concentration during interaction with these cells were determined spectrophotometrically. The dependence of the reaction velocity on the original bilirubin concentration followed an adsorption isotherm, typical of enzyme processes with aK m of 79 μm at a cell concentration of 500/νL. The primary event is the adsorption of bilirubin to the cell surface and, in addition, its detoxication. Cyanide and sonic treatment of cells inhibit the reaction, salicylic acid enhances the cell activity. The bilirubin-detoxicating effect is discussed with respect to the therapy of hyperbilirubinaemia in neonates.
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The authors would like to express sincere thanks to I. Machuldv’a for skilled techmical assistance
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Knobloch, E., Miler, I. Interaction of bilirubin with human lymphocytes and granulocytes — the effect on bilirubin metabolism. Folia Microbiol. 31, 387–393 (1986). https://doi.org/10.1007/BF02936604
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DOI: https://doi.org/10.1007/BF02936604