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Roles of glucose and acetate as carbon sources inl-histidine production withBrevibacterium flavum FERM1564 revealed by metabolic flux analysis

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Abstract

The metabolic flux pattern forl-histidine production was analyzed when glucose and/or acetate were used as carbon sources. Totall-histidine production was enhanced when mixed substrate (glucose and acetate) was used, compared with that when either glucose or acetate was used as the sole carbon source. Theoretical maximum carbon fluxes through the main pathways forl-histidine production, cell growth, and ATP consumption for cell maintenance were obtained by the linear programming (LP) method. By comparison of the theoretical maximum carbon fluxes with actual ones, it was found that a large amount of glucose was actually used for maintenance of cell viability. On the other hand, acetate was used for cell growth. After depletion of acetate in the mixed substrate culture, the flux for glucose tol-histidine synthesis was markedly enhanced. A strategy for effectivel-histidine production using both carbon sources was proposed.

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Abbreviations

r i :

mole flux (i=1…13) (mole/h)

r maxi :

theoretical maximum flux (mole/h)

f i :

percentage of mole flux in three main pathways (%)

His/Gluc:

histidine synthesis from glucose

X/Gluc:

biomass synthesis from glucose

mATP/Gluc:

ATP consumption for cell maintenance from glucose

His/Ac:

histidine synthesis from acetate

X/Ac:

biomass synthesis from acetate

mATP/Ac:

ATP consumption for cell maintenance from acetate

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Authors and Affiliations

  1. Department of Biotechnology, Graduate School of Engineering, Osaka University, 565-0871, Osaka, Japan

    Hiroshi Shimizu, Nobuyuki Shimizu & Suteaki Shioya

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  1. Hiroshi Shimizu
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  2. Nobuyuki Shimizu
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  3. Suteaki Shioya
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Correspondence to Suteaki Shioya.

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Shimizu, H., Shimizu, N. & Shioya, S. Roles of glucose and acetate as carbon sources inl-histidine production withBrevibacterium flavum FERM1564 revealed by metabolic flux analysis. Biotechnol. Bioprocess Eng. 7, 171–177 (2002). https://doi.org/10.1007/BF02932915

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  • DOI: https://doi.org/10.1007/BF02932915

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