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Antibody-quantum dot conjugates exhibit enhanced antibacterial effectvs. unconjugated quantum dots

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Abstract

The effect of a 20-min exposure to antibody-quantum dot (Ab-QD) conjugates on colony counts ofEscherichia coli was assessed by the spread-plate method and compared with exposure to unconjugated QDs having only amine or carboxyl groups on their surfaces. Under these conditions, Ab-QD conjugates generally exhibited >90% reduction in colony-forming units as compared to untreatedE. coli andE. coli treated with unconjugated QDs after incubation for as long as 41 h. The antibacterial effect of Ab-QD conjugatesvs. unconjugated QDs onSalmonella enterica subsp.enterica serovar Typhimurium was also assessed by means of a disk-diffusion technique which demonstrated greater growth inhibition (≈3 mm greater) by Ab-QD conjugate-impregnated disks than by unconjugated-QD-only-impregnated disks at a 10-µg disk load. At a 25-µg disk load, both treatment groups exhibited nearly equal growth inhibition.

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Abbreviations

Salmonella Typhimurium:

Salmonella enterica subsp.enterica serovar Typhimurium

Ab:

antibody

Ab-QD:

antibody-quantum dot (conjugate)

AG:

Adirondack Green (QD)

FO:

Fort Orange (QD)

IgM:

immunoglobulin M

QD:

quantum dot

pb:

p between groups

AMP:

adenosine monophosphate

CFU:

colony-forming unit

PBS:

phosphate-buffered saline

TSA:

Tryptic soy agar

TSB:

trypticase soy broth

p w :

p within groups

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Authors and Affiliations

  1. NanoScience Diagnostics Inc., 78758, Austin, TX, USA

    S. Dwarakanath & P. Rao

  2. Operational Technologies Corporation, 78229, San Antonio, TX, USA

    J. G. Bruno

  3. Department of Microbiology and Biotechnology, Bangalore University, 560 056, Bangalore, India

    T. N. Athmaram & G. Bali

  4. Texas State University-San Marcos, 78666, San Marcos, TX, USA

    D. Vattem

Authors
  1. S. Dwarakanath
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  2. J. G. Bruno
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  3. T. N. Athmaram
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  4. G. Bali
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  5. D. Vattem
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  6. P. Rao
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Corresponding author

Correspondence to J. G. Bruno.

Additional information

This work was funded in part byUS Army contract no. DACA42-03-C-0063.

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Dwarakanath, S., Bruno, J.G., Athmaram, T.N. et al. Antibody-quantum dot conjugates exhibit enhanced antibacterial effectvs. unconjugated quantum dots. Folia Microbiol 52, 31–34 (2007). https://doi.org/10.1007/BF02932134

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  • DOI: https://doi.org/10.1007/BF02932134

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