Abstract
Oligosaccharides produced by submerged cultures ofC. africana andC. sorghi were isolated by semipreparative HPLC. Structure of 6-O-β-d-fructofuranosyl-d-glucopyranose (blastose), 1,6-bis-O-(β-d-fructofuranosyl)-α-d-glucopyranoside (neokestose) and two sugar alcohols, 1-O-β-d-fructofuranosyl-d-mannitol (fructosylmannitol) and 1,6-bis-O-(β-d-fructofuranosyl)-d-mannitol (bisfructosylmannitol) was determined by NMR spectrometry. MALDI TOF MS analysis revealed molecular ions [M+Na]+ that indicate the presence of other tetra- and pentasaccharides (m/z=689.4 and 851.5, respectively) and corresponding sugar alcohol (m/z=691.4). Rapid conversion of sucrose into series of oligosaccharides and corresponding sugar alcohols was observed in all tested strains.
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We express our appreciation to Prof. P.G. Mantle for his gift of honeydewC. sorghi. The research was supported by theGrant Agency of the Czech Republic (grant 525/00/1283),Universities Development Fund (grant 2052/2001), and byInstitutional Research Concept AV 0Z 5020 0510.
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Flieger, M., Kantorová, M., Halada, P. et al. Oligosaccharides produced by submerged cultures ofClaviceps africana andClaviceps sorghi . Folia Microbiol 50, 198–204 (2005). https://doi.org/10.1007/BF02931566
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DOI: https://doi.org/10.1007/BF02931566