Abstract
The lead phosphate precipitation method showed the reaction product of acid phosphatase (which reflects the presence of the enzyme glycoprotein) in peripheral cytoplasmic vesicles in the ascomycetous fungusClaviceps purpurea. The product appeared to diffuse from these vesicles (diameter 100–200 nm) towards the cell wall, usually to its sites covered by the capsular fibres exhibiting also acid phosphatase activity. This observation of diffusion of secretory glycoprotein in the cytoplasmic matrix and its orientation to the plasmalemma and capsular fibrils suggests an alternative to the well-described secretory mechanism of transport and exocytosis of glycoproteinsvia membrane-bound transport conveyors fusing with the cell membrane. It confirms and enlarges our previous finding of the reaction product of acid phosphatase performed by ultrastructural cytochemistry in vacuoles (lysosomes), in the growing cell septum, in cytoplasmic vesicles and in the fibres of the external capsule (Voříšek J.:Arch.Microbiol. 111, 289–295, 1977).
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References
Hislop E.C., Barnaby V.M., Shellis C., Laborda F.: Localization of α-l-arabinofuranosidase and acid phosphatase in mycelium ofSclerotinia fructigena.J.Gen.Microbiol. 81, 79–99 (1974).
Hugon J.L.: Lead salt methods, pp. 29–58 in E. Wisse, W.T. Doems, I. Mollenauer, P. Van Duijn (Eds):Electron Microscopy and Cytochemistry. North Holland Publishers, Amsterdam 1973.
Ketchum J.C., Schmidt W.K., Rajendrakumar G.V., Michaelis S., Maloney P.C.: The yeast a-factor transporter Ste6p, a member of the ABC superfamily, couples ATP hydrolysis to pheromone export.J.Biol.Chem. 276, 29007–29011 (2001).
Řeháček Z., Sajdl P., Kozova J., Malik K.A., Řičicová A.: Correlation of certain alterations in metabolic activity with alkaloid production by submergedClaviceps.Appl.Microbiol. 22, 949–956 (1971).
Van Rijn H.J.M., Linnemans W.A.M., Boehr P.: Localization of acid phosphatase in protoplasts fromSaccharomyces cerevisiae.J.Bacteriol. 123, 1144–1149 (1975).
Voříšek J.: Electron-cytochemical demonstration of acid phosphatase in saprophyticClaviceps purpurea.Arch.Microbiol. 111, 289–295 (1977).
Voříšek J.: Functional morphology of the secretory pathway organelles in yeast.Microscopy Res.Techn. 51, 530–546 (2000).
Voříšek J., Lojda Z.: Electron-microscopic localization of acetyl coenzyme A carboxylase in cells ofClaviceps pupurea.Histochemistry 65, 59–65 (1979).
Voříšek J., Gas N., Denis-Duphil M.: Cerium-based ultracytochemical localization of aspartate transcarbamylase activity in the cell membrane complex ofSaccharomyces cerevisiae.Micron 28, 221–230 (1977).
Voříšek J., Knotková A., Kotyk A.: Fine cytochemical localization of polyphosphates in the yeastSaccharomyces cerevisiae.Zbl. Microbiol. 137, 421–432 (1982).
Voříšek J., Techniková Z., Schwippel J., Benoist P.: Enzymatic activities of Ura2 and Ura1 proteins (aspartate transcarbamoyltransferase and dihydroorotate dehydrogenase) are present in both isolated membranes and cytoplasm ofSaccharomyces cerevisiae.Yeast 19, 449–457 (2002).
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Voříšek, J., Kalachová, L. Secretion of acid phosphatase inClaviceps purpurea — an ultracytochemical study. Folia Microbiol 48, 767–770 (2003). https://doi.org/10.1007/BF02931511
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DOI: https://doi.org/10.1007/BF02931511