Abstract
Bacterial isolatesComamonas terrigena N3H (from soil contaminated with crude oil) andC. testosteroni (isolated from the sludge of a wastewater treatment plant), exhibit much higher total catalase activity than the same species from laboratory collection cultures. Electrophoretic resolution of catalases revealed only one corresponding band in cell-free extracts of bothC. testosteroni cultures. Isolates ofC. terrigena N3H exhibited catalase-1 and catalase-2 activity, whereas in the collection cultureC. terrigena ATCC 8461 only catalase-1 was detected. The environmental isolates exhibited much higher resistance to exogenous H2O2 (20, 40 mmol/L) than collection cultures, mainly in the middle and late exponential growth phases. The stepwise H2O2-adapted culture ofC. terrigena N3H, which was more resistant to oxidative stress than the original isolate, exhibited an increase of catalase and peroxidase activity represented by catalase-1. Pretreatment of cells with 0.5 mmol/L H2O2 followed by an application of the oxidative agent in toxic concentrations (up to 40 mmol/L) increased the rate of cell survival in the original isolate, but not in the H2O2-adapted variant. The protection of bacteria caused by such pretreatment corresponded with stimulation of catalase activity in pretreated culture.
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Abbreviations
- EEP:
-
early exponential phase
- LSP:
-
late stationary phase
- LEP:
-
late exponential phase
- MEP:
-
middle exponential phase
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This work was supported by VEGA grant of theSlovak Academy of Sciences no. 2/5069/25.
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Godočíková, J., Boháčová, V., Zámocký, M. et al. Production of catalases byComamonas spp. and resistance to oxidative stress. Folia Microbiol 50, 113–118 (2005). https://doi.org/10.1007/BF02931458
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DOI: https://doi.org/10.1007/BF02931458