Abstract
S. cerevisiae growth and responses to different treatments were monitored by two-dimensional fluorescence spectroscopy, which simultaneously detects the fluorescence of a number of cells’ own fluorophores. Growth curves of cultures of free cells were measured by means of tryptophan fluorescence in nonfluorescent culture medium and a flow-through system at a suitable excitation/emission beam geometry. Fast responses of the cells to anaerobic-aerobic transition or addition of glucose, methanol or cyanide, which could not be measured in this system because of the time delay inherent in transporting the cells from the culture flask to the cuvette, were monitored with cells immobilized in alginate. The major fluorescence changes caused by these treatments belonged to NAD(P)H which is a good indicator of the redox state of the cells.
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The work was supported by grant S 502 0202 of theGrant Agency of the Academy of Sciences of the Czech Republic, by grant ME 577 of theMinistry of Education. Youth and Sports of the Czech Republic and byInstitutional Research Concept no. AV 0Z 502 0903.
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Podrazký, O., Kuncová, G., Krasowska, A. et al. Monitoring the growth and stress responses of yeast cells by two-dimensional fluorescence spectroscopy: First results. Folia Microbiol 48, 189–192 (2003). https://doi.org/10.1007/BF02930954
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DOI: https://doi.org/10.1007/BF02930954