Abstract
A biosensor to quantify L-proline within 10-5–10-3 mole/L concentration is described. ImmobilizedPseudomonas sp. cells grown in a medium containing L-proline as the only source of carbon and nitrogen were used to create the biosensor. The cells oxidized L-proline specifically consuming O2 and did not react with other amino acids and sugars. The change in oxygen concentration was detected with a Clark oxygen membrane electrode. The cells were immobilized by entrapment in polyvinyl alcohol (PVA) cryogel. The resultant biocatalyst had a high mechanical strength and retained its L-proline-oxidizing ability for at least two months.
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Simonian, A.L., Rainina, E.I., Lozinsky, V.I. et al. A biosensor for L-proline determination by use of immobilized microbial cells. Appl Biochem Biotechnol 36, 199–210 (1992). https://doi.org/10.1007/BF02921779
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DOI: https://doi.org/10.1007/BF02921779