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Cell division and chromosome numbers in the tissue culture ofNicotiana tabacum

Buněčné dělení a počty chromòsomů ve tkáňových kulturáchNicotiana tabacum

Клеточное деление и хромосомном наборе в культуре тканейNicotiana tabacum

  • Published:
Biologia Plantarum

Abstract

The callus tissue derived from tobacco pith and subcultured for 51/2 years on a solid synthetic modium revealed considerable differences in cell division activity depending on the age of the subculture and diurnal rhythm. The callus cells exhibited different level of ploidy among which an aneuploid condition nearer to diploid (2n=30–50) predominated. Chromosomal bridges and other structural rearrangements (lagging chromosomes, fragments) were observed in ana-and telophase.

Abstract

V kalusové tkáni ze dřeně tabáku, kultivované 51/2 roku na pevném synthetickém médiu, so projevovaly značné rozdíly v aktivitě buněčného dělení v závislosti na stáří kultury a denním rytmu. Kalusové buňky vykazovaly různý stupeň ploidie, kde převládal charakter aneuploidní blízký diploidnímu (2n=30–50). V anafázi a telofázi byly pozorovány chromosomové můstky a různé strukturální změny (opožděné chromosomy, fragmenty).

Abstract

Каллусная ткань, изолированная от сердцевинь табака и культивированая 5,5 лет на агаровой синтетической питательной среде, обнаруживала заметнье различия в активности клеточных делений в зависимости от возраста пассажа и суточного цикла. Клетки каллуса имели разную степень плоидности с преобладанием анеуплоидного состояния (2r=30–50). В ана-и телофазах заметили хромосомнье мость и другие аберрапии (отставание хромсом, фрагменть).

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Authors and Affiliations

  1. Department of Genetics and Darwinism, Tartu State University, Tartu

    H. Kallak

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  1. H. Kallak
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Address: Botanic Garden, Tartu, Estonian S.S.R.

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Kallak, H. Cell division and chromosome numbers in the tissue culture ofNicotiana tabacum . Biol Plant 10, 199–204 (1968). https://doi.org/10.1007/BF02921037

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  • DOI: https://doi.org/10.1007/BF02921037

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