biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 36:335, 1994 | DOI: 10.1007/BF02920927

Effect of 2,4-dichlorophenoxyacetic acid on nuclear division in stem segments ofPisum sativum L. cultivatedin vitro

L. Gantchev1, W. Bleiss1
1 FB Biologie, Humboldt-Universität zu Berlin, Berlin, Germany

We have investigated the influence of 2,4-dichlorophenoxyacetic acid (2,4-D) on the appearance of nuclear fragments, caused by direct nuclear division, as well as on mitotic activity in cultivated internodial stem segments ofPisum sativum L., cv. Bördi, during 180 d of cultivation. Direct nuclear fragmentation (dNF) was indicated by the shape and structure of the nucleus as well as by the occurrence of 1C- and 3C-values of DNA, investigated cytophotometrically. The dNF occurred during the whole cultivation period in segments treated by 2,4-D in concentrations from 4 to 32 mg 1-1. In the presence of 2 mg 1-1 of 2,4-D the dNF existed in the explants only up to 90 d. Mitotic activity was not observed in the 2,4-D-free control but occurred during the whole cultivation period when 2,4-D was added in concentrations from 2 to 16 mg l-1. In the presence of 32 mg l-1 of 2,4-D the level of mitotic activity was very low at the beginning and ceased after 60 d in culture.

Received: September 1, 1993; Accepted: November 9, 1993; Published: September 1, 1994  Show citation

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Gantchev, L., & Bleiss, W. (1994). Effect of 2,4-dichlorophenoxyacetic acid on nuclear division in stem segments ofPisum sativum L. cultivatedin vitro. Biologia plantarum, 36(3), 335. doi: 10.1007/BF02920927
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