Skip to main content
Log in

Peroxidase, polyphenol oxidase and catalase isoenzymes during germination and early plant development of tall and dwarf wheats (Triticum aestivum L.)

  • Brief Communication
  • Published:
Biologia Plantarum

Abstract

Isoenzyme pattern of peroxidase, polyphenol oxidase and catalase at different intervals during germination and in different plant parts during early development of ‘C-306’ and ‘Hira’ cultivars, representing tall and dwarf wheats, respectively were investigated. The number of isoenzymic components of the three enzymes increased progressively with the concomitant increase in the stage of germination. The isoenzymic patterns, in general, were more clear and better developed during the later stages of germination. Different tissues or organs of the wheat plant possessed specific isoenzymic pattern of these enzymes. Differences between cultivars were observed both in the number and relative intensity of the various isoenzymic fractions.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

  • Davis, B. J.: Disc electrophoresis. II. Method and application to human serum proteins.—Ann. N. Y. Acad. Sci.121: 404–427, 1964.

    Article  CAS  PubMed  Google Scholar 

  • Evans, J. J., Alldridge, N. A.: The distribution of peroxidases in extreme dwarf and normal tomato (Lycopersicon esculentum Mill.)—Phytochemistry4: 499–503, 1965.

    Article  CAS  Google Scholar 

  • Grommeck, R., Markakis, P.: Effect of peroxidase on anthocyanin pigments.—J. Food Sci.29: 53–57, 1964.

    Article  CAS  Google Scholar 

  • Kamerbeek, G. A.: Peroxidase content of dwarf types and giant types of plants.—Acta bot. Neerl.5: 257–263, 1956.

    Article  CAS  Google Scholar 

  • Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, H. J.: Protein measurement with the Folin—phenol reagent.—J. biol. Chem.193: 265–275, 1951.

    CAS  PubMed  Google Scholar 

  • Macko, O. H., Honold, G. R., Stahmann, M. A.: Soluble proteins and multiple enzyme forms in early growth of wheat.—Phytochemistry6: 465–471, 1967.

    Article  CAS  Google Scholar 

  • McCune, D. C., Galston, A. W.: Inverse effects of gibberellin on peroxidase activity and growth in dwarf strain of pea and corn.—Plant Physiol.34: 416–418, 1959.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Ross, H.: Über die Verschiedenheiten des dissimilatorischen Stoffwechsels in reziprokenEpilobium-Bastarden und die physiologisch-genetische Ursache der reziproken Unterschiede, I. Die Aktivität der Peroxidase in reziprokenEpilobium-Bastarden mit der Sippe Jena.—Z. Ind. Abst. U. Vererb. Lehre.82: 503–529, 1941.

    Google Scholar 

  • Scandalios, J. G.: Genetic control of multiple molecular forms of enzymes in plant.—Biochem. Genet.3: 37–39, 1969.

    Article  CAS  Google Scholar 

  • Schertz, K. F., Nadine, A. S., Igor, V., Sarkissian, Hart, G. E.: Peroxidase regulation by three dwarf height locus in sorghum.—J. Hered.62: 235–241, 1971.

    CAS  Google Scholar 

  • Shin, M., Nakamura, W.: Indole acetic acid oxidase activity of wheat peroxidase.—J. Biochem.52: 444–448, 1962.

    CAS  PubMed  Google Scholar 

  • Siegel, S. M.: The biosynthesis of lignin: Evidence for the participation of cellulose as sites for oxidative polymerization of eugenol.—J. amer. chem. Soc.78: 1753–1755, 1956.

    Article  CAS  Google Scholar 

  • Siegel, S. M.: Non-enzymic macromolecules as matrices in biological synthesis. The role of polysacharides in peroxidase catalysed lignin polymer formation from eugenol.—J. amer. chem. Soc.79: 1628–1632, 1957.

    Article  CAS  Google Scholar 

  • Siegel, B. Z., Galston, A. W.: The isoperoxidase ofPisum sativum.—Plant Physiol.42: 221–226, 1967.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Singh, R., Ahlawat, T. R.: Substrate specificity and inhibition of wheat tyrosinase.—Indian J. exp. Biol.11: 397–399, 1973.

    CAS  Google Scholar 

  • Singh, R., Sheoran, I. S.: Enzymic browning of whole wheat meal flour.—J. Sci. Food Agr.23: 121–125, 1972.

    Article  CAS  Google Scholar 

  • Vanlear, D. H., Smith, W. H.: Soluble proteins and enzyme pattern in shoots of slash pine under different nutritional regimes.—Phytochemistry9: 1929–1932, 1970.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Singh, R., Singh, D. Peroxidase, polyphenol oxidase and catalase isoenzymes during germination and early plant development of tall and dwarf wheats (Triticum aestivum L.). Biol Plant 17, 235–240 (1975). https://doi.org/10.1007/BF02920846

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02920846

Keywords

Navigation