Abstract
The paper describes isolation and partial characterization of the tissue culture of the common spruce (Picea excelsa Link). The tissue culture was grown on the medium ofHarvey (1967) modified by increasing the concentration of 2,4-dichlorophenoxyacetic acid (2,4-D) to l mg l−1 and by omitting the tyrosine. By selective 10-month subculturing the tissue culture was obtained possessing relatively stable properties and giving good yields of biomass. The tissue of the callus was of yellowish colour, compact and homogenous. The obtained tissue culture was characterized by its growth curve and by following the O2 consumption during the growth.
Abstract
V tejto práci sa popisuje izolácia a čiastočná charakterizácia pletivovej kultúry smreku obyčajného (Picea excelsa Link), ktorú sme kultivovali na upravenej pôde podlaHarveya (1967), zvýšením koncentrácie 2,4-dichlórfenoxyoctovej kyseliny na 1 mg/l a vynechaním tyrozínu. Selektívnym 10-mesačným pasážovaním sa nám podarilo získat kultúru s pomerne stálymi vlastnostami a dobrými výtažkami bunečnej hmoty. Kalusové pletivo je nažltlej farby, kompaktné a homogénne. Získanú pletivovú kultúru sme čiastočne charakterizovali rastovou krivkou, spotrebou O2 počas rastu a % sušiny.
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Hricová, D., Strmeň, J. & Bauer, Š. Preparation and characterization of the tissue culture of spruce (Picea excelsa Link). Biol Plant 16, 118–122 (1974). https://doi.org/10.1007/BF02920786
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DOI: https://doi.org/10.1007/BF02920786