Abstract
CV1 and A549 cells were grown in the presence of64Cu porphyric complex,64CuCl2, or67CuCl2. Radioactive copper determinations were performed on whole cells and on isolated cellular DNA.125IUdR was used to calibrate the particular extraction and purification procedures we developed because of the half-lives of64Cu and67Cu. The results obtained have shown that some radioactive copper atoms remained firmly bound to the DNA molecule. Their amount was of the same order when using two different DNA isolation methods for the two cell lines studied. No significant differences were found when64Cu was used as CuCl2 or as porphyric complex.
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Grisvard, J., Guille, E., Sasaki, I. et al. Biochemical studies of human (A549) and Simian (CV1) cells labeled with64Cu,67Cu, and125IUdR. Biol Trace Elem Res 20, 207–224 (1989). https://doi.org/10.1007/BF02917436
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DOI: https://doi.org/10.1007/BF02917436