Abstract
The capacity of blood and lymphatic cells and of the spleen in rabbits immunized with sheep red blood cells to produce antibodies (PFC) has been studied. In rabbits in which only the blood was examined, the numbers of PFC reached a peak 4 days after one immunization, or 3 days after two immunizations. Moreover, double immunization yielded 10 times higher numbers of PFC than single immunization. Comparative studies of the blood, lymph and spleen revealed largest numbers of PFC in the spleen, smaller in the blood, and fewest in the lymph. This pattern was observed after single as well as double immunization, although the proportions were different after double immunization. After the second immunization much larger numbers of PFC appeared, increasing especially in the lymph. Discrepancies between the findings and earlier results, and the problem of the mechanisms of permeation of immunologically competent cells into the blood are discussed.
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Adler, F. L.:Studies on mouse antibodies. I. The response to sheep red cells. J. Immunol. 95: 26, 1965.
Cunningham, A. J., Smith, J. B., Mercer, E. H.:Antibody formation by single cells from lymph nodes and efferent lymph of sheep. J. exptl. Med. 124: 701, 1966.
Gowans, J. L., Knight, E. J.:The route of recirculation of lymphocytes in the rat. Proc. roy. Soc. London, Series B. 159: 257, 1964.
Halasa, J.:Antibody producing alveolar cells. I. Plaque forming cells among alveoral cells among alveoral cells after intravenous and intratracheal immunization. (In Polish).Archivum Immunologiae Therapiae Experimentalis. 16:29, 1968.
Halasa, J.:Antibody producing alveolar cells. II. The effect of inhalation of antigen in rabbits previously immunized intravenously (In Polish).Archivum Immunologiae et Therapiae Experimentalis. 16: 40, 1968.
Hull, J. G., Morris, B., Moreno, G. D., Bessis, M. C.:The ultrastructure and function of the cells in lymph following antigenic stimulation. J. exptl. Med. 125: 91, 1967.
Hull, J. G., Morris, B.:The origin of the cells in the efferent lymph from a single lymph node. J. exptl. Med. 121: 901, 1965.
Hulliger, L., Sorkin, E.:Synthesis of antibodies by Blood leucocytes of the rabbit. Nature (Lond.). 198: 299, 1963.
Hulliger, L., Sorkin, E.:Formation of specific antibody by circulating cells. Immunology, 9: 391, 1965.
Jerne, N. K., Nordin, A. A., Henry, C:The agar plaque technique for recognizing antibody-producing cells. Cell-bound antibodies, Philadelphia 1963.
Jerne, N. K.:Studies on the primary immune cellular response in mice. Molecular and cellular basis of antibody formation. Ed. J. Šterzl. p. 459 Czech. Acad. Sci., Prague, 1965.
Kearney, R., Halliday, W. J.:Enumeration of antibody-forming cells in the peripheral blood of immunized rabbits. J. Immunol. 95: 109, 1965.
Landy, M., Sanderson, R. P., Bernstein, M. T., Jackson, A. L.:Antibody production by leucocytes in peripheral blood. Nature (Lond.) 204: 1320, 1964.
Landy, M., Sanderson, R. P., Jackson, A. L.:Humoral and cellular aspects of the immune response to the somatic antigen of Salmonella enteritidis. J. exptl. Med., 122: 483, 1965.
Medlín, J. personal communication.
Sorkin, E.:Cell-bound and cell-binding antibody in the peripheral blood of immunized rabbits. Nature (Lond.). 204: 794, 1964.
Sorkin, E., Landy, M.:Antibody production by blood leucocytes. Experientia, 23: 677, 1965.
Šterzl, J., Mandel, L.:Estimation of the inductive phase of antibody formation by plaque technique. Fol. microbiol. 9: 173, 1964.
Sercarz, E. E., Byers, V. S.:The X—Y—Z scheme of immunocyte maturation. III. Early IgM memory and the nature of the memory cell. J. Immunol. 98: 836, 1967.
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Hałasa, J. Plaque forming cells in the spleen, lymph and blood. Folia Microbiol 13, 253–258 (1968). https://doi.org/10.1007/BF02909610
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DOI: https://doi.org/10.1007/BF02909610